You cut the signal-anchor sequence out of the cDNA for a type II membrane protein and...
You cut the signal-anchor sequence out of the cDNA for a type II membrane protein and express it in cultured human cells and then perform immunofluorescent staining. Describe where this protein should normally be found and where your mutated protein will be found.
Homework Answers
Type II membrane proteins are single-pass membrane proteins, it does not have an N terminal signal sequence, these proteins have a signal-anchor sequence, which will be inserted into the ER membrane, then finally end ups in the plasma membrane, that is normal protein is found in plasma membrane.
If the signal-anchor sequence is removed from the cDNA and expressed in cells, the mRNA produced from this insert lacks signal-anchor sequence, so the ribosome translates the mRNA in the cytosol only, the mRNA-ribosome complex is not translocated to the ER membrane, so the protein will be translated to cytosol and protein remains in cytosol, so mutated protein can be found in the cytosol.
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