Question

Bacillus amy 1. Which of the following bacteria was the source of the restriction enzyme Sall 2. Below is the recognition loliquefaciens H site for Sall. When the DNA B. globigii was cut at this site, would the Escherchia coli RY13 cut have sticky or would the cut have blunt ends? Haemophilus influenzae Rd H. parainfluenzae Klebsiella pneumoniae OK 8 S-GTCGAC-3 3-CAGCTG-5 Streptomyces albus Staphylococcus aureus 3A1 3. What is the term for the study in which DNA is extracted from an environment and all of it is sequenced? 1. CRISPR is an acronym for: 5. Cas9 is an acronym for:

Answer 1

Question 1

Answer: Sal1 is an restriction enzyme that cleave the DNA at restriction site 5′-G/TCGAC-3′ . The source of Sal1 is Streptomyces albus G.

Question 2

Answer: When the DNA is cut with Sal1 restriction enzyme, sticky ends are produced because the enzyme produces 5' and 3' overhangs in the DNA strand.

Question 3

Answer: Metagenomics is the term used to study DNA recovered from environmental samples using DNA sequencing. The DNA extracted from the environmental samples is sequenced using sequencing platforms and the sequences were analyzed using bioinformatics programs.

Question 4

Answer: CRISPR is the acronym for Clustered Regularly Interspaced Short Palindromic Repeats. These are the DNA sequences found in the bacteria and achaea. They are used in genome editing.

Question 5

Answer: Cas9 is the acronym for CRISPR-associated protein 9 . It is an enzyme that recognizes and cleaves specific sequence of DNA complementary to CRISPR sequence. Cas 9 uses CRISPR sequences as a guide. This enzyme play a major role in genome editing.