5. In five sentences, summarize how an ELISA assay "works." 6. How is the test quantified?
Explain how a pregnancy test works and how it is a variation of the ELISA technique.
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
1. What does an ELISA test for? 2. What variables can affect the outcome? (Name 4) 3. What are the three important limitations of an ELISA? Explain each. 4. In the virtual lab, the ELISA plate has been pretreated to bind SLE antigen to each well. WHY? How do you expect the assay setup to differ in our case where the antigen of interest is present in a heterogenous sample in small quantities? 5. Why are there both a positive...
Summarize in a paragraph of 5 to 6 sentences the mission of the IFRS. (5 pts.) What is the IASB, what is its vision and how many members does it have? Indicates the name of the current chairman. (5 pts.) What are the two main functions of the IFRS Foundation? Explain briefly. (5 pts.)
As a bulleted list, using one to three complete sentences, summarize five things you learned from reading chapter one of the textbook. Do NOT answer based on class notes or non-textbook readings and do not guess. Chapter one from Animal Physiology, 4th ed. Hill, Richard W., Wyse, Gordon A., & Anderson, Margaret. Sinauer Press: 2016. ISBN-10: 1605355941 (3rd edition works too).
5. Explain the rapid diagnostic test, the ELISA, or the neutralization test. What are the pros and cons of each test?
1. Describe how a ChIP assay works: • What basic steps are involved in a ChIP assay? • What 3 (or 4) important pieces of information can this assay provide about a protein? • What are the limitations of this technique (eg. resolution)? • What sort of controls should be included and why?
Each paragraph will be no less than 3 sentences and no more than 6 sentences. Summarize your understanding of the organization of the body from the simplest elements to the more complex.
1a. In 3 to 5 sentences, highlight what a splay tree is and how it works. 1b. In natural language, describe how to carry out the following operations in a splay tree. i. search ii. insert iii. delete
Choose a television commercial and try to summarize it. Don't use more than five sentences. Did you have any difficulties? If so, what were they? Remember that the two discussions in this module will be graded together. For more information, please refer to the Discussion Rubric Link