Question

A CHO mammalian cell culture is set up by a chemostat stirred tank bioreactor to produce HBsAg. Glucose is the growth rate limited substrate and cell growth follows the Monod Model. The feeding flow rate is 20L/hr with the glucose concentration 0.25M, and the glucose concentration in the outlet is 5g/L. It has been found out the maximal cell growth rate is 0.2 hr-1 and the saturation constant, or half-velocity constant of substrate is 2.0 g/L. The cell yield coefficient is Y(X/S) = 0.30g/g. Find out:

a)CHO cell concentration in the outlet stream (7 marks)

b)Culture medium volume in the bioreactor at flow rate of 20L/hr (6 marks)

c)Keep the culture medium volume from b) constant, what is maximal flow rate to avoid the cells washed out from this bioreactor? (7 marks)

Answer 1

we know H 't follows monal to uster we 나 M и U=D D-v VOL. Data we hove. SAO Sol From monod kinectics that - ИСА. CMICA To determine whether Kinches find Plot blano См. I uc Line Comes If Straight thon model is followed state At steady Obtining A plotting R=0.99 y = 24188744106419 Model is followed toia Cm=0.609 X4 1.687= 1850389 mg/l 3. now too foo Vo = 74.hu X=0.9. Cao = Joomg/L. Coco. Balonce on Elhonol VoCo - voln - M G V =o. Cro-CAM CA T. Croten CA = Cno (1-0.6)= Joox 0.1= 90 mg/L. k=0-609 hil = 1-6419 Too

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using mass balance on chemostat we can find the required variables by using monods kinetics

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