Question

help me answer quetion 1 please

11 42% & 17:19 4/ Homework 2- ECH160,pdf Quarter 2, 2018-2019 ECH 160Homework 2 Instructor: N.T Linh, Dept. OR&P, HUMG Assigned on 24 May, 2019 Due on 27 May, 2019 Problem 1 A mouse-mouse hybridoma cell line is used to produce monoclonal antibody. Growth in batch culture is monitored with the following results. Time (day) Cell concentration (cells/ml) x 10 0.0 0.45 0.2 0.52 0.65 0.5 0.81 1.5 1.22 1.77 2.13 3.0 3.55 4.02 3.5 3.77 4.0 2.20 4.5 (a) Determine the specific growth rate during the growth phase. (b) What is the culture doubling time? Problem 2 Zymomonas mobilis is used to convert glucose to ethanol in a batch fermenter under anaerobic conditions. The yield of biomass from substrate is 0.06 g/g: YPx is 7.7 g/g. The maintenance coefficien s 2.2 gg.h; the specific rate of product formation due to maintenance is . h. The maximum specific growth rate of Z. mobilis is approximately 0.3h. 5g bacteria are inoculated a 60 m fermenter of medium containing 12 g/L glucose, K, for the organism is 0.2 g/L a) Determine batch culture times required to produce 10 g biomass? b) What flow rate is required for a steady-state substrate concentration of 1.5 g/L? c) At the flow rate of (b), what is the cell density? d) At the flow rate of (b), what concentration of ethanol is produced?

Answer 1

DATE Time nimher of gencratian 1 eneration Time Daubling Time tion loa Ccells at the end-Ina Gellt at beginning/ gowth ph aue of growth ph aue .301 log s 2.20)-l0g (0.45) 6 30 5 27さ 6 generation Daubling time 10& Chr) 6480 min 1080 cells Amin

x2.303 cells