Question

Answer the following two questions (i,ii) about this figure.

On plasmid 1 (gene encoding): Gal4 BD YFHP Start with a strain containing reporter gene and plasmid 1, transform with cDNA fusion library in plasmid 2: On plasmid 2 (gene encoding): HcDNA library Gal4 AD HSubunit clone Gal4 AD Random Hclone Gal4 AD GROWTH on Minimal Media lacking Histidine NO Growth on Minimal Media lacking Histidine

i. Where is this assay taking place?

A. In vitro

B. in yeast cells

C. in drosophila tissue culture cells

D. in C. elegans (worms) tissue culture cells

E. in human tissue culture cells

ii. What does the H in HcDNA library stand for?

A. Histone

B. His3

C. Human

D. Haploid

E. Harvested

Answer 1

i) The Gal4 transcription factor is present in yeast cells.

So, the correct answer is option (B).

ii) H in HcDNA library stands for Human. ie, Human cDNA library.

So, the correct answer is option (C)