Cas9 has two catalytic domains (HNH & RuvC) that perform DNA cleavage - one domain per...
Cas9 has two catalytic domains (HNH & RuvC) that perform DNA cleavage - one domain per strand of DNA. At the chemical level, are these two domains catalyzing DNA cleavage in a similar fashion to each other? What are the similarities or differences?
Homework Answers
Cas9 contains two magnesium-dependent nuclease domains: an HNH domain that cleaves the complementary (target) strand of the DNA target, and a RuvC domain responsible for cleavage of the displaced noncomplementary (non-target) DNA strand.
The RuvC domain is encoded by sequentially disparate sites that interact in the tertiary structure to form the RuvC cleavage domain.
Despite low sequence similarity, the sequence similar to RNase H has a RuvC fold (one member of RNase H family) and the HNH region folds as T4 Endo VII (one member of HNH endonuclease family).
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Cas9 has two catalytic domains (HNH & RuvC) that perform DNA
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hi! it has to be answered in the format shown above the question. so you say...
hi!
it has to be answered in the format shown above the question. so
you say the correct answer but then you go through each one and say
why the other choices are incorrect one by one.
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BIU A A E 8. A DNA molecule has two strands (double helix) – how many...
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help me answer these- One strand of a section of DNA Isolated from the bacterium E....
help me answer these-
One strand of a section of DNA Isolated from the bacterium E. cow reads: answer all parts 5- GTAAGCTACGGATAGG -3 A. Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template meaning this is the coding strand). What will be the sequence of the mRNA in this region (make sure you label the 5 and 3' ends of the mRNA)? B. How many different peptides could potentially be made from...
1. Describe one experiment that can test the hypothesis that DNA replication is semi conservative. Describe...
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es and that they 7) Suppose that one human rypote has the normal number of DNA...
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Double stranded DNA consists of two long strands. An adenine on one strand is always paired to a thymine on the opposite strand and a guanine on one strand is always paired to a cytosine on the opposite strand. If a purified DNA has 36% thymine, what arc the percentages of the other bases? A solution has 10 mu g/L of enzyme X, how much enzyme X is in each of the following amounts of solution. 100 mL 50 mL...
hi!
it has to be answered in the format shown above the question. so
you say the correct answer but then you go through each one and say
why the other choices are incorrect one by one.
conservative fashion C) Synthesis of the lagging strand requires more helicase enzymes than synthesis of the leading strand, D) The lagging strand has a higher mutation rate than the leading strand. The correct answer is A. Answer B is wrong because DNA replication...
BIU A A E 8. A DNA molecule has two strands (double helix) – how many of its strands are/is used during replication and transcription? 9. What is the difference between transcription and translation occurring in Prokaryotes and eukaryotes? 10. What is the relation between mRNA and genetic code? 11. Using genetic code table-list the corresponding aminoacids for triplet code: CAG, GAG, CAC, AUA, GUA, GCA and UGA, UAA and UAG 12. How many start and stop codons are there?...
help me answer these-
One strand of a section of DNA Isolated from the bacterium E. cow reads: answer all parts 5- GTAAGCTACGGATAGG -3 A. Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template meaning this is the coding strand). What will be the sequence of the mRNA in this region (make sure you label the 5 and 3' ends of the mRNA)? B. How many different peptides could potentially be made from...
es and that they 7) Suppose that one human rypote has the normal number of DNA proofreading and repair enzymes and that the function correctly, while another, for some reason, has no such functioning enzymes. As growth and developmen. proceeded, what differences in DNA structure in the cells and overall organism functioning would you expect to f... between the two? 8) In what normal human cells does telomerase function? In what disease does telomerase function in the cells (much to...
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