Question

TASK II DNA Sequence DNA was also collected (Table 1). You are going to use this DNA sequence to complete the assignment Sacher, tochemelebiT.CO. DA A CARTIE CURIE CRET GARAGE MATIC I GREW E G CARETTING GUITAR HET GRATUIT TE GOOIT GENIET CARTIE CUCINE ACRET GARAGE GOTE GOOIT GRONIE I The DNA examined is 100 nucleotides of mitochondrial sequence, specifically the cytochrome Coxidase subunit I (MT-001) gene. This gene is often used as a DNA barcode to identity/differentiate animals at the species level. The MT-COi gene is often used for this level of phylogenetic examination because it has a fast mutation rate, but is conserved among conspecifics. Below is the character matrix constructed from the nucleotide sequence above. Note the positions where one or more of the in-group tau have a different nucleotide than the out-group The DNA examined is 100 nucleotides of mitochondrial sequence, specifically the cytochrome Coxidase subunit I (MT-C01) gene. This gene is often used as a DNA barcode to identity/differentiate animals at the species level. The MT-001 gene is often used for this level of phylogenetic examination because it has a fast mutation rate, but is conserved among conspecifics. Below is the character matrix constructed from the nucleotide sequence above. Note the positions where one or more of the in-group taxa have a different nucleotide than the out-group. Nucleotide Position Taxa 10 25 59 68 72 с A с т G A с А T с A с 0 A B E G H I G G с A A A A A A A А G G G с A A т G Create the most parsimonious DNA sequence tree for the taxa above. This is a clearer view of the first table below. chondrial DNA sequerytochrome Celebenik OT.CO. DA елностте екосяким менин минекекет алматамен тенист еконати скламите селянин незаметне CATCHTHAT THEY GANCHAINCANTATGE EAGLETEC GARA GARATGE TI Table 0 A E F смокттын самситм контети какожкет емислимен статтест семиналите климат жените текстие скокти салсактат кактикт аксесст аласымен астаттест GEORGE GOGATATTI CARTUCCERTAT ATT ACET ACCANCE TEATRE CARAM GOCAGGA CARTE ANGKAT TROET GAROTA TERET MART COM GASTATUT H 1

Answer 1

Hey man, I couldn’t make the phylogenetic tree since you didn’t provide much information to propose a molecular clock hypothesis and also the whole DNA sequences you were working with are missing; so I used the ones in the character matrix constructed from the nucleotide sequences to create a similarity matrix (according to the number of positions with different nucleotides of each sequence and relative to the O sequence (also called external group)) and after that I created the following cladogram.

	O	A	B	C	D	E	F	G	H	I
O	0
A		0	1							2
B		1	0							1
C				0	0	2
D				0	0	2
E				2	2	0
F							0	1	2
G							1	0	2
H							2	2	0
I		2	1							0

This is a part of the distance matrix I constructed; I don’t show all values (the important ones are highlighted) since when you calculate it you can see there are 3 particular groups always formed (you can see this much better in the character matrix you provided), these are: A-B-I, C-D-E and H-G-F, the position of the branches that connects them variates A LOT and that’s the reason why a bunch of trees can be fitted to a particular dataset.

This is the final cladogram:

G F о DE А в 1 с т