Homework Answers
Structural regions in lac operon is in the following order.Lactose ang Glucose are the major payers in lac operon regulation.
CAP site-Promotor region-Operator-lac Z-lac Y-lac-A.
CAP site is where Catabolite Activator Protein(CAP) binds in regulating Operon.When CAP binds to CAP site it allows transcription to takes place.When cAMP binds to CAP,CAP binds to CAP region.
| + | - | - | + | off |
cAMP is formed only when glucose levels is low.Binding of CAP is needed to have high levels of transcription.
RNA polymerase binds to Promotor region.
Repressor molecule binds to Operator region when Lactose is not present.So,it prevents RNA polymerase going head.Then,it will be in OFF state.
When Lactose is present,lactose binds to repressor molecule ,thus making repressor inactive.Therefore Operator region is is free from repressor.RNA polymerase moves forward and it allows transcription.
Thus genes Z,Y,A are expressed which allows the bacterial cell to metabolise Lactose.
| Glucose | Lactose | CAP binding | Repressor binding to Operator | Operon ON/OFF |
| + | - | - | + | off |
| - | + | + | - | ON |
| + | + | - | - | off(very low transcription) |
| - | - | + | + | off |
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