13) EMB agar is selective for gram negative bacteria as the methylene blue dye present in the media is toxic and inhibits the growth of gram positive bacteria.
14) Yes, the bacteria grown on this culture plate is capable of fermenting lactose to result in acidic by products that reduce the pH of the medium thus changing the colour of the indicator (methylene blue) in the media.
Please answer all 3) The bacteria cultured on the surface of this eosin methylene blue agar...
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What type(s) of bacteria are favored to grow on eosin methylene blue (EMB) agar? Select one: O a Gram positive bacteria that can undergo anaerobic respiration. O b. Gram negative bacteria that can ferment glucose and/ or lactose, O c. Only those bacilli that can utilize citrate as a main carbohydrate source. O d. All Bacteria would grow equally well A bacterial species grows on a MacConkey agar plate, and the colonies have dark red color with hazy...
1)An eosin methylene blue (EMB) agar plate was inoculated with four different species of bacteria. Below are the colony growth after inoculation. Which shows a vigorous lactose fermenter? A) A brown media with an oblong culture of bacteria. The colony shows vigorous growth and is the same color as the media. B) A brown media with an oblong colony of bacteria that experiences weak growth. The color of the media is visible through the translucent white color of the bacterial...
Multiple Choice. Highlight the single correct answer choice. 1. Streak plates are useful in microbiology to __________. quantify the number of bacteria measure turbidity identify bacteria determine cell shape 2. In the streak-plate technique, the intent is to isolate bacteria by dilution in theory by __________. dilution on a solid surface separating cells within the solid surface using a pipette dilution in water blanks 3. A pure culture consists of which of the following? one genus of microbe one species...
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(1 pt 27) The two bacteria cultured on this mannitol salt agar plate will have the same reaction. One reacted nearly 24 hours before the other. What substance in the agar was catabolized by both bacteria that produced the demonstrated colorimetric change? Image gallery (click for full size) (1pt 28) The presence of bacterial growth on this mannitol salt agar plate suggests that these bacteria can tolerate the hyperosmotic condition produced by the salt. The term used...
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(1 29) The bacteria cultured on the left side of this phenol red dextrose agar plate metabolically converted what substance in this medium to create the demonstrated colorimetric change? Image gallery (click for full size) (1 30) The bacteria on the right side of this phenol red dextrose agar plate metabolically converted what substance in this medium to create the demonstrated colorimetric change (be specific)? Image gallery (click for full size)
BI0S242, Week 7, Lab 1 2. Do all the bacteria samples grow on Eosin Methylene Blue and MacConkey agar? not, why What does a metallic green sheen indicate on an Eosin Methylene Bhue plate? 3. 4. What additional information did the lactose fermentation tubes provide? Why couldn't we examine any anaerobic bacteria that are part of the normal digestive system? 5. 6. Were there any colonies in the rest room samples? Could you identify any that gave similar results on...
can somrone help me write an introduction for each agar. thank you
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Introduction: Selective and differential media are used frequently in Microbiology Pathology Labs to identify a known group of bacteria from a complex mix of other bacteria. The medium is designed to select against as many of the background organisms as possible by using a medium in which they will not grow but the organisms under investigation can grow hence the "Selective" part of the name. Any assayable...
4-6 Eosin Methylene Blue Agar (Levine) OBSERVATIONS AND INTERPRETATIONS Refer to Table 4-6, page 268, when recording your results and interpretations in the table below. Use abbreviations or symbols as needed. Growth (N/P/G) EMBINA Organism EMB Growth Color (Pi/D/C) Interpretation and Presumptive ID QUESTIONS I in your own words, what is the application (purpose) of EMB agar? 2 Which ingredient(s) in EMB agar supply(ies) a. Carbon b. Nitrogen? ur choice and explana 3 Is EMB agar a defined or an...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such as A. to inhibit motility B. to stain microbes C to kill bacteria D. to see microbes to detect pH changes due to metabolism What is the purpose of differential medias to encourage growth of the desired bacteria B to isolate few bacteria to observe metabolism of the bacteria D. to stain bacteria E to determine motility 10 Why does Staphylococcus aureus turn yellow...
Background
Ques part 1)
Selective and differential media; EMB and Tsl agar bacill inhabit a wide range of environments. Many are significant pathogens, such a coli, Salmonella, Shigella, but many compose our normal flora and inhabit soil and water. Many gra negative bacilli that inhabit our large intestine are called enterics. Enteric pathogens are the most frequent cause of diarrheal illnesses and have become significant contaminants spread through foo Some can also cause respiratory and urinary infections. Experiment 1: EMB...