Question

Please answer all

8) The exoenzyme produced by Bacillus subtilis for the hydrolysis of starch (1pts) is: caseinase. gelatinase diastase. lactomase. (1 pts) 9) In anaerobic conditions, the end products of carbohydrate respiration are alcohols, organic acids and perhaps carbon dioxide; while, the aerobic end products are water, carbon dioxide and perhaps ammonium. O True O False 10) Antimicrobial activity, as demonstrated by the Kirby-Bauer Method of antibiotic efficacy testing, (1 pts) is indicated by the evolution of gas. the zone of inhibition. the minimal inhibitory concentration the phenol coefficient. a change in pH. O the McFarland Scale. (1 pts) 11) Which of the following substances is not a source of carbon? ammonium salt Olipids O polysaccharides proteins starch

Answer 1

8)

Answer: diastase

The bacteria can utilize starch as an energy source. But for that, it has to break down the starch into glucose molecules so that it can enter into the bacterial cell for further metabolism. To do so, it releases the exoenzyme that hydrolyzes starch into glucose monomers.

Caesinase is a protease that hydrolyzes the protein caesin. It does not hydrolyze starch which is a carbohydrate.

Gelatinase is another protease that hydrolyzes gelatin into its sub-compounds. It does not hydrolyze starch which is a carbohydrate.

There exists no enzyme by the name lactomase reported in scientific literature. However, there is an enzyme called lactamase that hydrolyzes the beta-lactams of penicillim. It does not hydrolyze starch.

9)

Answer: False

During the aerobic respiration of carbohydrates, ammonium is released at not step.

10)

Answer: the zone of inhibition

In Kirby-Bauer's disk diffusion test, the efficacy of an antibiotic is tested by measuring the size of the zone upto which the growth of bacteria has been inhibited in a culture plate.

All other options are incorrect becase none of those parameters are measured in the Kirby-Bauer's test.

11)

Answer: Ammonium salts

Ammonium salts are inorganic compounds, Thus, they consist of inorganic elements and cannot act as a source of carbon (which is organic).

Lipids, starch, polysaccharides and proteins, all are organic compounds that have a carbon skeleton in their structure and can thus act as carbon sources.

______________________________________

[Kindly give a thumbs up]