Question

Question 4 You have been asked by your supervisor to help with their study on a tumor suppressorgen in humans Shown below is the structure of a human tumor suppressorgee divided into 10 se designated A-L. The gene contains three exons, two intron, a promoter, and a site in for poly(A) addition Expn 1 Ex Promoter Iron Iron MIA a) What segment or segments of the gene will be represented in the newly transcribed RNA transcript! List in order, the appropriate lener or letters (1 mark) b) What seement or segments of the gene will be found in the completely processed materemRNA transcript What segment or segments of the gene in the processed mature mRNA transcript wil hovedditional nucleotides added to them? (1 mark) 4) What segmente segments of the gene contain the TATA box clement? mark) c) What segment o segments of the gene will possess the translation initiatiecode? (lmark) Loss-of-function mutations in tumor suppresser goes are common in many human cancers. A class of loss of function mutations are called mull mutations, and all allele is a mutants of the completely lacks that gene's normal function. This can be the result of the express of action product (RNA or protein) or the complete absence of the gene product (RNA or protein Your supervisor is interested in the areas of the gene where mutations would have the greatest effect on phenotype. You are presented with some hypothetical situations of the human mappe gee. In a large sample of nullalkekes, would you expect there to be mutant sites in segment (mark) mutant sites do exist, would you expect the transcript to be detected Explain marks) Would you expect a polypeptide chain to be producer Explain.marks) e) In a large sample of nullalleles, would you expect there to be mutant sitesinement DF (ark) mutant sites de exist would you expect the transcript to be detected Explain marks Would you expect a polypeptide chain to be produced? Explain marks)

Answer 1

4.a. In the newly transcribed pre-mRNA transcript the gene segments present are DEFGHI.

b. In completely processed mRNA DFHI will be found.

c. I will have additional nucleotides added to them to make the polyA tail longer.

d. C contains the TATA box element.

e. D will possess the translation initiation codon.

f. Yes there can be mutations in segment C. If there is mutation in the promoter high chance is there that transcript will not be detected. This is because if there is mutation in the promoter the polymerase cannot bind and start transcription from the promoter. If transcription doesn't happen translation will also not happen because the mRNA sequence gets translated to protein.

g. Yes segment D can get mutated. If it is mutated then transcript will be produced as the promoter is functional and RNA polymerase can bind to it and cause transcription. Polypeptide chain will be produced unless and until mutation results in the replacement of a codon to a stop codon.