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3) (10 marks) Acetylcholinesterase catalyzes the hydrolysis of the neurotransmitter acetylcholine: Acetylcholine + H2O → acetate...
The enzyme that hydrolyzes acetylcholine to form acetate and choline has a Km = 9 x 10-5 M for the substrate. In an reaction flask with 5 nanomol / mL of the enzyme and 150 µM of acetylcholine, an initial reaction rate of 40 µmol / mLs was observed. a) Calculate vmax for this amount of enzyme. b) Calculate kcat for the enzyme. c) Calculate the catalytic efficiency of the enzyme. d) Does this enzyme approach “catalytic perfection?
b) (10 points) Acetylcholinesterase is a hydrolase that hydrolyzes the neurotransmitter acetylcholine. Caffeine inhibits the action of Acetylcholinesterase and clinical studies have indicated that it can be involved in the slowing of Alzheimer disease pathology. P11149 is a galanthamine analogue that has also been studied as a potential drug for treating Alzheimer disease. The initial reaction rate for acetylcholine hydrolysis was measured at an enzyme concentration of 5×10-8 M with no inhibitor present, and in the presence of caffeine or...