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Is it possible a bacterium that can only grow at the bottom of a tube of thioglycolate broth is also oxidase-positive? Why, o
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Answer b. No, it is not possible. It is an obligate anaerobe, and have no reasons to have enzymes needed for aerobic respiration

Thyoglycolate broth is an enriched medium used in microbiology to determine the oxygen requirements of microorganisms. The organisms that grow at the bottom of the tube in thyoglycolate broth are obligate anaerobes.

The oxidase test is used for detecting the presence of the terminal enzyme system in aerobic respiration called cytochrome C oxidase. Being obligate anaerobes, they are devoid of oxidase enzyme because they metabolise energy anaerobically.

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