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Why is us hard to determine out what sperm and egg proteins are interactinf dueing membrane...

Why is us hard to determine out what sperm and egg proteins are interactinf dueing membrane fusion by mammals during feetilization?

Why is it difficult to determine what sperm and egg proteins are interacting during membrane fusion of mammalian fertilization.
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Actually the process of fertilization is not a one step or single phase process it is a multi phase process and in this processes many proteins as well as enzymes are involved and all this happens invivo in a cascade process.

Now if we can imagine the complexity of this multi-phase cascade process we can clearly realize that this process can not be properly understand from out side.

Another most important part is that the participating cells the male and female gamete (sperm & egg) both are so tiny that er need microscope to visualize them.

So in this scenario it is really difficult to understand what sperm and egg proteins are interacting during membrane fusion of mammalian fertilization.

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