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The results of the following unknown bacteria sample would be: A B. Select one: a. Oxidase positive and catalase positive b.
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Answer- c. Oxidase positive and catalase negative.

Oxidase test is used to determine whether bacteria produce cytochrome c oxidase or not. This test is to be done to identify the bacteria from genus Neisseria and genus Pseudomonas. For oxidase test, with swab dipped in reagent tetra- methyl-p- phenylenediamine dihydrochloride scrap colonies of bacteria, if purple to black colour generates on swab means bacteria is oxidase positive and no colour change means bacteria is oxidase negative.

Catalase test is used to identify the bacteria that produces enzyme catalase. This enzyme acts on hydrogen peroxide detoxifies it and break it into water and oxygen gas. If bacteria is catalase positive, production of oxygen gas bubbles are clearly seen.

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