Homework Answers
1. what is sequence of DNA templete strand from gel ?
From the given picture ( if pictures are taken properly without
repeating bands on the gel ) following is the sequence.
5' TGTCGCCGCAGAAT 3'
the number of bands in the given gel is 14 and according to bands
number of purine and pyrimidine is 14. on the gel, the direction of
electrophoresis is negative to positive ( large fragments to short
fragments) but the direction of sequence read is opposite from
positive to negative so this is actually 5' to 3'.
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