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QUESTION 31 The gel below shows the result of sequencing a piece of DNA (the template). Each lane shows the fragments of th
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1. what is sequence of DNA templete strand from gel ?

From the given picture ( if pictures are taken properly without repeating bands on the gel ) following is the sequence.

5' TGTCGCCGCAGAAT 3'

the number of bands in the given gel is 14 and according to bands number of purine and pyrimidine is 14. on the gel, the direction of electrophoresis is negative to positive ( large fragments to short fragments) but the direction of sequence read is opposite from positive to negative so this is actually 5' to 3'.

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