Homework Answers
Plaque forming units (Pfu).
Plaque forming units is the measure of number of infectious virus particles present in a suspension. Each plaque on the plate denotes a single viirus particle.
Higher the Pfu, the higher the number of virus particles in a suspension, the higher the lysis.
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What is the term for the number of viral particles in a suspension? O plaque forming...
What is the term for the number of virions released from an infected host cell? plaque...
What is the term for the number of virions released from an infected host cell? plaque forming unit O virus forming unit O phage forming unit O burst size O blowout size
genetics Select the definition of a bacteriophage plaque. O O O O the bacteriophage capsid a...
genetics
Select the definition of a bacteriophage plaque. O O O O the bacteriophage capsid a bacterial cell producing more bacteriophage a visible region of infection-induced lysis of bacterial cells a bacteriophage colony If a single phage starts a replication cycle that results in 34 viable phage, how many phage can be produ after 3 replication cycles? Number phage
finish the chart please and question in the end Dilution Plaque Number PFU/mL | 1:1,000 |...
finish the chart please and question in the end
Dilution Plaque Number PFU/mL | 1:1,000 | 1:10,000 | 1:100,000 >2,000 200 T20 For the PFU/mL calculation, only plates giving 25-250 plaques should be used. For the plaque number, indicate when the bacterial cells are cleared due to too many phage particles in the infection. What is the number of phage particles in the original phage stock?
You repeat the viral plaque assay adding 10 microliters of viral solution to 990 microliters of...
You repeat the viral plaque assay adding 10 microliters of viral solution to 990 microliters of LB. You perform this serial dilution five times. You add 10 microliters from each dilution to the corresponding host bacteria molten top agar mixture which is then spread onto an LB agar plate. The next day you count 39 plaques on the fourth plate. Why is it that on plate 3 the number of plaques will be 39 x 10^2 and on plate 5...
The Term "Titer" in Medicine refers to the Concentration of Antibody Molecules or Virus Particles as...
The Term "Titer" in Medicine refers to the Concentration of Antibody Molecules or Virus Particles as determined by a Dilution Series. The Titer of our fX174 Bacteriophage is usually ~ 1.0 times 10^10 Plaque Forming Units per Milliliter (pfu/ml), indicating that there are at least 1.0 times 10^10 Bacteriophage per Milliliter. Assuming that our fX174 Bacteriophage Titer actually is 1.0 times 10^10 Pfu/ml, and assuming that each Bacteriophage actually produces a Plaque, approximately how many Plaques should you expect to...
Strain R of E. coli has an RM system that modifies/cleaves the sequence GAATTC. Sometimes invading...
Strain R of E. coli has an RM system that modifies/cleaves the sequence GAATTC. Sometimes invading phage DNA is methylated by the strain R methylase before the endonuclease can cleave the viral DNA, and then replicates in the methylated form and forms viral particles. If you add some of these viral particles to uninfected bacteria of strain R, what will happen? Select one: a. The invading phage DNA is unmethylated and is protected from the strain Rendonuclease b. The invading...
The concentration of particles in a suspension is 48 per mL. A 5 mL volume of...
The concentration of particles in a suspension is 48 per mL. A 5 mL volume of the suspension is withdrawn. a. What is the probability that the average number of particles per mL in the withdrawn sample is between 46 and 50? b. If a 10 mL sample is withdrawn, what is the probability that the average number per mL of particles in the withdrawn sample is between 46 and 50? c. How large a sample must be withdrawn so...
The number of viral particles in a preparation can be indirectly determined using a hemaglutination assay....
The number of viral particles in a preparation can be indirectly determined using a hemaglutination assay. a. True b. False Moving to another question will save this response
recombination in phages you have isolated two mutations that affect plaque morphology in phages (y and...
recombination in phages
you have isolated two mutations that affect plaque
morphology in phages (y and z). you conduct a genetic cross between
the two phage strains by coinfecting bacteria with a wild-type
strain and a strain that carries both mutations. phage chromosomes
enter the host and replicate. you collect some of the progeny
phages and plate them onto a lawn of bacterial cells. you observe
the following numbers of plaques:
1. which are examples of recombinant progeny?
Select all...
Question 51 Not yet answered Strain R of E. coli has an RM system that modifies/cleaves...
Question 51 Not yet answered Strain R of E. coli has an RM system that modifies/cleaves the sequence GAATTC. Sometimes invading phage DNA is methylated by the strain R methylase before the endonuclease can cleave the viral DNA, and then replicates in the methylated form and forms viral particles. If you add some of these viral particles to uninfected bacteria of strain R, what will happen? Points out of 2.50 P Flag question Select one: O a. The invading phage...
What is the term for the number of virions released from an infected host cell? plaque forming unit O virus forming unit O phage forming unit O burst size O blowout size
genetics
Select the definition of a bacteriophage plaque. O O O O the bacteriophage capsid a bacterial cell producing more bacteriophage a visible region of infection-induced lysis of bacterial cells a bacteriophage colony If a single phage starts a replication cycle that results in 34 viable phage, how many phage can be produ after 3 replication cycles? Number phage
finish the chart please and question in the end
Dilution Plaque Number PFU/mL | 1:1,000 | 1:10,000 | 1:100,000 >2,000 200 T20 For the PFU/mL calculation, only plates giving 25-250 plaques should be used. For the plaque number, indicate when the bacterial cells are cleared due to too many phage particles in the infection. What is the number of phage particles in the original phage stock?
The Term "Titer" in Medicine refers to the Concentration of Antibody Molecules or Virus Particles as determined by a Dilution Series. The Titer of our fX174 Bacteriophage is usually ~ 1.0 times 10^10 Plaque Forming Units per Milliliter (pfu/ml), indicating that there are at least 1.0 times 10^10 Bacteriophage per Milliliter. Assuming that our fX174 Bacteriophage Titer actually is 1.0 times 10^10 Pfu/ml, and assuming that each Bacteriophage actually produces a Plaque, approximately how many Plaques should you expect to...
Strain R of E. coli has an RM system that modifies/cleaves the sequence GAATTC. Sometimes invading phage DNA is methylated by the strain R methylase before the endonuclease can cleave the viral DNA, and then replicates in the methylated form and forms viral particles. If you add some of these viral particles to uninfected bacteria of strain R, what will happen? Select one: a. The invading phage DNA is unmethylated and is protected from the strain Rendonuclease b. The invading...
The number of viral particles in a preparation can be indirectly determined using a hemaglutination assay. a. True b. False Moving to another question will save this response
recombination in phages
you have isolated two mutations that affect plaque
morphology in phages (y and z). you conduct a genetic cross between
the two phage strains by coinfecting bacteria with a wild-type
strain and a strain that carries both mutations. phage chromosomes
enter the host and replicate. you collect some of the progeny
phages and plate them onto a lawn of bacterial cells. you observe
the following numbers of plaques:
1. which are examples of recombinant progeny?
Select all...
Question 51 Not yet answered Strain R of E. coli has an RM system that modifies/cleaves the sequence GAATTC. Sometimes invading phage DNA is methylated by the strain R methylase before the endonuclease can cleave the viral DNA, and then replicates in the methylated form and forms viral particles. If you add some of these viral particles to uninfected bacteria of strain R, what will happen? Points out of 2.50 P Flag question Select one: O a. The invading phage...
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