Question

Dilution Plaque Number PFU/mL | 1:1,000 | 1:10,000 | 1:100,000 >2,000 200 T20 For the PFU/mL calculation, only plates giving 25-250 plaques should be used. For the plaque number, indicate when the bacterial cells are cleared due to too many phage particles in the infection. What is the number of phage particles in the original phage stock?

finish the chart please and question in the end

Answer 1

The table and the relevant information provided by you is somewhat insufficient for completing the question. The total volume of the original phage stock and the volume plated on the petri plate is not given. I can elaborate the procedure and methodology to solve this problem, you just need to insert the missing information for getting the final answer.

Number of phage particles in original solution can be calculated by utilizing either 1:10,000 dilution or 1:100,000 dilution. I will use 1:10,000 dilution.

Dilution Factor = 10,000 = 10^4

For original solution :

PFU/mL = Plaque Number x Dilution Factor

= 200 x 10^4

= 2 x 10^6

= 2,000,000

Now, number of phage particles in original phage stock can be calculated by multiplying this PFU/mL with the total volume of the phage stock (in mL) , because the total volume is not given in question.

Now, calculating the PFU/mL for each dilution:

For 1:1,000 dilution -

Dilution Factor = 1000 = 10^3

Since Plaque Number falls outside the given range of 25-250, so we cannot determine the PFU/mL in this case.

For 1:10,000 dilution -

PFU/mL = Plaque Number / Volume Plated (in mL)

= 200 / Plated Volume

Insert the missing volume here, which was plated on petri plate to get the result.

For 1:100,000 dilution -

PFU/mL = Plaque Number / Volume Plated (in mL)

= 20 / Plated Volume

Insert the missing volume here, which was plated on petri plate to get the result.

This is how the problem is supposed to be solved.

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