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invas (2014) You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation. These

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The most directly affected part of the DNA replication process,if a strain of bacteria lacking helicase were used to make the cell extracts is Initiation of DNA synthesis.

DNA helicase initiates the first step of DNA replication by unzipping the DNA strands at the site of replication fork.It unwinds the two strands by breaking the hydrogen bonds and initiates replication.

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