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After observing your adherent cells in the T75 flask under the microscope, you estimate the confluence of the cells to be aroHow many total cells would you place in a T75 flask with a cell yield of 8.4 x 106 cells to seed the cells at 20% confluenceYou have cells at 80% confluence in a 175 flask and you want to seed as many 12-well plates as possible at a density of 2 x 1Continuing from the previous question... 1. How would you resuspend the cells from the 175 after centrifugation to obtain a r

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Answer #1

# 1. Cell count from the hemocytometer = 6.5 x 106.

Actual capacity of T75 flask = 8.4 x 106

Percentage of the cells = (6.5 x 106/8.4 x 106) X 100 = 77.3%

2. Hemocytometer is the more accurate method beacuse, we count the cells mannually.

3. Hemocytometer is the best method in determining the cell confuency whille seeding cells.

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