Helpppp Your cells are ready to split and you prepare to scale-up. Write a protocol for...
After observing your adherent cells in the T75 flask under the microscope, you estimate the confluence of the cells to be around 70%. When you passage the cells, you count the cells using a hemocytometer and obtain a total cell count of 6.5 x 10 cells. (T75 cell yield = 8.4 x 106 cells) 1. What does the cell count (from the hemocytometer) suggest is the % confluence of the flask? 2. Which number would you trust to be more...
Your cell-preparation protocol calls for you to prepare EXACTLY 1000 mL of dilute room temperature phosphate buffer solution. You use boiling water to quickly dissolve your phosphate salt. You carefully measure out exactly 1000 mL of this solution when it is still at 55∘C. Once the buffer solution has cooled to room temperature (20∘C), how much more or less volume solution do you actually have? (Be careful with your signs! Remember that a positive difference will be an increase in...
Biology Lah 10. (20) You are setting up an experiment to electroporate AX2 cells with a new vector. The vector has a Belomycin resistance marker gene. The literature tells you that Bleomycin is effective on dicty in a concentration range of 1-50 μg/ml in HL-5 media and cells die after 1-5 days. Your boss tells you that you need to determine the best concentration of Bleomycin to kill Dicty before you begin your electroporation experiment. How would you set up...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a previous experiment, you used polymerase chain reaction (PCR) to amplify a sequence that you believe to regulate expression of a gene you are studying. You will now take this purified PCR product (double stranded DNA) and ligate it into a plasmid that contains a luciferase reporter gene. If your DNA sequence is a promoter sequence, then its presence will allow for expression of the...
1) Your lab partner made three 20 mL volumes of penicillin at the following concentrations: 15 mg/mL, 18 mg/mL, and 45 mg/mL. Your lab protocol requires a large amount of 25 mg/mL penicillin, so you decide to combine all of the three solutions to make as much at that concentration as possible. How much 25 mg/mL penicillin can you make? How much water will you need to add? 2) You started with a cell count of 6.5 x 105 cells...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
Critical Thinking Question (5 pts.) (You may use the back of the page to write your answer if needed) Today you have learned about DNA, the cell cycle, chromosomes, and the special end-tips of the chromosomes called Telomeres. Each time a cell divides the Telomeres get shorter and shorter, until they get so short that a cell stops dividing and eventually dies This is a part of normal aging process. We know that stress can speed up the shortening of...
E and F only thank you 16. The concentration of TnC in muscle cells is estimated at about 70 nmol g' wet weight of tissue. 02 consumption is estimated to be about 0.09 mL O2 per m in per g wet weight of tissue. Assume the TnC is 60% saturated during normal, resting contractions. Assume that 80% of the activating Ca originates from SR stores. Assume a heart rate of 70 min. Assume free energy of ATP hydrolysis is 57...
Your role as spokesperson is to write and present a prepared statement and prepare responses to no less than five questions you may be expecting from the media Your company recently released its latest version of a popular business and industry software program. Programs always have a few bugs or problems upon release, even after the most rigorous laboratory testing, but this program is apparently infested with bugs. Stories are popping up across the Internet about how the program does...
9. In human cells four different cyclins drive the cell cycle (see picture). Please, note that the cyclins overlap (the picture shows the concentration of each cyclin protein in the cytoplasm). Write a short essay or outline of an experiment to test the following things: How can you figure out how much cyclin protein is present in the cytoplasm at different phases of the cell cycle? B. How can you figure out if there is overlap between the cyclins? For...