Your cell-preparation protocol calls for you to prepare EXACTLY 1000 mL of dilute room temperature phosphate...
Your cell-preparation protocol calls for you to prepare EXACTLY 1000 mL of dilute room temperature phosphate buffer solution. You use boiling water to quickly dissolve your phosphate salt. You carefully measure out exactly 1000 mL of this solution when it is still at 55∘C. Once the buffer solution has cooled to room temperature (20∘C), how much more or less volume solution do you actually have?
(Be careful with your signs! Remember that a positive difference will be an increase in volume and a negative difference will be a decrease)
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Your cell-preparation protocol calls for you to prepare EXACTLY
1000 mL of dilute room temperature phosphate...
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
are there any synthetic transformations produced in this lab Step 1. Preparation and Recrystallization of a...
are there any synthetic transformations produced in this
lab
Step 1. Preparation and Recrystallization of a Diastereomeric Salt - Synthesis of (R,R)-1,2-Diammoniumcyclohexane mono-(+)-tartrate: In a 150 ml beaker, L-(+)-Tartaric acid (7.5 g, 0.05 mol) is dissolved in 25 mL of distilled water. The solution is stirred as 11.4 g (12.2 mL, 0.10 mol) of 1,2-diaminocyclohexane is added carefully in one portion. (The addition of the diamine is exothermic.) A slurry is formed initially but complete dissolution is observed once addition...
You are dissatisfied with the purity of the final preparation of your HRP and decided to...
You are dissatisfied with the purity of the final preparation of
your HRP and decided to include ion-exchange (IEC) and
gel-exclusion chromatography (GEC) steps in your purification
protocol. From the literature, you found out the following info
about HRP enzyme:
Molecular weight: 40,000
Isoelectric point: 7.2
HRP is a very stable protein.
2: Design GEC purification experiment using the information in
the appendix. Address and justify the following
I. choice of GEC column
II. choice of the buffer (if you...
Can you answer question 4 & 5? The experiment and porcedure is posted below Question 4...
Can
you answer question 4 & 5? The experiment and porcedure is
posted below
Question 4 & 5
CHM3120C Analytical Chemistry Lab Lab 7. Spectrophotometric Determination of Iron in Mohr's Salt with o-Phenanthroline Required Reagents and Supplies: 50 mL of 0.0005M -0.0006 M of Mohr's solution (unknown), 10 g of Hydroxylamine hydrochloride (NH2OH, HCI), 500 mL of o-phenanthroline solution (contains 0.5 g of o-phenanthroline monohydrate), 83 g of sodium acetate (NaOAc. 3H20) in 500 mL of water (for 1.2 M...
Preparation of Benzoic Acid using a Grignard Reagent URGENT 1. During your Grignard formation, a small...
Preparation of Benzoic Acid using a Grignard Reagent URGENT 1. During your Grignard formation, a small amount of benzene is formed. Provide a brief explanation and mechanism to explain this observation. 2. During your Grignard formation, a small amount of biphenyl is formed. Provide a brief explanation and mechanism to explain this observation. 3. What mass of water would be required to destroy the phenylmagnesium bromide that you prepared in this experiment? What volume does this represent? 4. Why is...
Principles of Inorganic Chemistry! Week 5 Effect of Temperature on Solubility of a Salt In this experiment, you w...
Principles of Inorganic Chemistry! Week 5 Effect of Temperature on Solubility of a Salt In this experiment, you will study the effect of changing temperature on the amount of solute that will dissolve in a given amount of water. Water solubility is an important physical property in chemistry, and is often expressed as the mass of solute that dissolves in 100 g of water at a certain temperature. In this experiment, you will completely dissolve different quantities of potassium nitrate,...
PREPARATION OF PHARMACEUTICALS - Fischer Esterification Reactions Q: Calculate the expected mass of both methyl salicylate and aspirin assuming 100% yield. EXPERIMENT7 PREPARATION OF PHARMACEUTICALS...
PREPARATION OF PHARMACEUTICALS - Fischer Esterification
Reactions
Q: Calculate the expected mass of both methyl salicylate and
aspirin assuming 100% yield.
EXPERIMENT7 PREPARATION OF PHARMACEUTICALS Fischer Esterification Reactions Almost 2500 years ago, physicians such as Hippocrates recommended that patients chew on the bark of the willow to alleviate pain. The active ingredient in willow bark was found to be salicin, a compound made of a molecule of salicyl alcohol bonded to a p-D-glucose molecule. In the stomach, the bond between...
1. Table 1 shows the temperature-time data were recorded for the reaction between 50.0 mL of 1.06 M HA (a we...
1. Table 1 shows the temperature-time data were recorded for the reaction between 50.0 mL of 1.06 M HA (a weak acid) and 50.0 mL of 0.90 MNH OH, ammonium hydroxide (a weak base, also known as aqueous ammonia) The solutions were mixed after 60 s of approximately constant temperature readings of 24.20°C. Table 1: Temperature-time Data Time (s) Temperature (C) Time (s) Temperature C) 31 90 24.25 30.9 105 24.22 15 120 30.8 24.20 30 30.7 135 24.20 45...
Write out your separation scheme for this experiment. 1. Heat 75 mL of water to 90...
Write out your separation scheme for this experiment. 1. Heat 75 mL of water to 90 °C in a 250-mL beaker. 2. While the water is being heated, place 250 mg of salicylic acid, 1 drop of 85% phosphoric acid and 0.5 mL of acetic anhydride in a test tube. Add a boiling chip to the mixture and gently shake it in order to mix the reactants. 3. Heat the tube in the beaker of water at 85 - 90...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
are there any synthetic transformations produced in this
lab
Step 1. Preparation and Recrystallization of a Diastereomeric Salt - Synthesis of (R,R)-1,2-Diammoniumcyclohexane mono-(+)-tartrate: In a 150 ml beaker, L-(+)-Tartaric acid (7.5 g, 0.05 mol) is dissolved in 25 mL of distilled water. The solution is stirred as 11.4 g (12.2 mL, 0.10 mol) of 1,2-diaminocyclohexane is added carefully in one portion. (The addition of the diamine is exothermic.) A slurry is formed initially but complete dissolution is observed once addition...
You are dissatisfied with the purity of the final preparation of
your HRP and decided to include ion-exchange (IEC) and
gel-exclusion chromatography (GEC) steps in your purification
protocol. From the literature, you found out the following info
about HRP enzyme:
Molecular weight: 40,000
Isoelectric point: 7.2
HRP is a very stable protein.
2: Design GEC purification experiment using the information in
the appendix. Address and justify the following
I. choice of GEC column
II. choice of the buffer (if you...
Can
you answer question 4 & 5? The experiment and porcedure is
posted below
Question 4 & 5
CHM3120C Analytical Chemistry Lab Lab 7. Spectrophotometric Determination of Iron in Mohr's Salt with o-Phenanthroline Required Reagents and Supplies: 50 mL of 0.0005M -0.0006 M of Mohr's solution (unknown), 10 g of Hydroxylamine hydrochloride (NH2OH, HCI), 500 mL of o-phenanthroline solution (contains 0.5 g of o-phenanthroline monohydrate), 83 g of sodium acetate (NaOAc. 3H20) in 500 mL of water (for 1.2 M...
Principles of Inorganic Chemistry! Week 5 Effect of Temperature on Solubility of a Salt In this experiment, you will study the effect of changing temperature on the amount of solute that will dissolve in a given amount of water. Water solubility is an important physical property in chemistry, and is often expressed as the mass of solute that dissolves in 100 g of water at a certain temperature. In this experiment, you will completely dissolve different quantities of potassium nitrate,...
PREPARATION OF PHARMACEUTICALS - Fischer Esterification
Reactions
Q: Calculate the expected mass of both methyl salicylate and
aspirin assuming 100% yield.
EXPERIMENT7 PREPARATION OF PHARMACEUTICALS Fischer Esterification Reactions Almost 2500 years ago, physicians such as Hippocrates recommended that patients chew on the bark of the willow to alleviate pain. The active ingredient in willow bark was found to be salicin, a compound made of a molecule of salicyl alcohol bonded to a p-D-glucose molecule. In the stomach, the bond between...
1. Table 1 shows the temperature-time data were recorded for the reaction between 50.0 mL of 1.06 M HA (a weak acid) and 50.0 mL of 0.90 MNH OH, ammonium hydroxide (a weak base, also known as aqueous ammonia) The solutions were mixed after 60 s of approximately constant temperature readings of 24.20°C. Table 1: Temperature-time Data Time (s) Temperature (C) Time (s) Temperature C) 31 90 24.25 30.9 105 24.22 15 120 30.8 24.20 30 30.7 135 24.20 45...
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