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DISCUSSION POST #2 If bacteria are grown on plates with ampicillin and X-gal, explain which color colonies, white or blue, woJustify please!

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Selection of Recombinants due to inactivation antibiotics is a difficult and time-consuming process as it requires simultaneously plating on two different plates having different antibiotics.

Alternative selectable markers developed which help in identifying the recombinants from non-recombinants on their ability to produce color in the presence of a chromogenic substrate. In this method,a recombinant DNA is inserted within the coding sequence of an enzyme which is referred to as insertional inactivation. The presence of a Chromogenic substrate X-gal gives blue colored colonies if the plasmid in the bacteria does not have an insert.

Presence of insert results into insertional inactivation of the Beta-galactosidase (reporter enzyme)and the colonies do not produce any color. These white color colonies are identified as recombinant colonies.

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