Question

isdR thr eu azi lon proa Pura PYTB lac Isx purd argE malk mel pure tha lip pola 06 THE H ily T108d1 gal chie Hfr Hfr cl Pyre mti Hit KL25 08 20 xy pisB Hfr AB313 Hfr KL.191 (Hir G11 Hfr 87) mall st 70 30 Hir KL 14 argo % THtr KL.16 argG Hlr KL98) Her KL961 40 man tolc metc serA aroD 50 pabB his UVIC

Using the figure as a guide, for a mating experiment between Hfr KL98 and F- auxotrophic recipient, a defined medium lacking which amino acid would use to select for recipients?

a. histidine

b. threoanine

c. arginine

d. proline

e. tryptophan

Answer 1

Answer is b. Threoanine.

The lacking amino acid used to select for recipients is threoanine.

Sample placed in blender. Two strains At 1-minute intervals, grown in non samples agitated to selective liquid separate conjugating medium. cells. Cells plated onto medium containing streptomycin, which kills original donor cells, and lacking threonine to select against nonmated F cells. Azide o Bacteriophage T1 Lactose Galactose Replica plating transfers each colony to media that select for four donor markers other than streptomycin.