please select answer from options given in [] and are bold, there are 3 parts 1....
please select answer from options given in [] and are bold, there are 3 parts
1. An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1.0, 3.0 and 5.0 kb are seen.
2. When the same plasmid is cut with both the EcoRI and BamHI restriction enzymes (a double digest), bands of 1.0, 1.5, 3.0, and 3.5 kb are seen.
From these results, it can be concluded that ["EcoRI", "BamHI"] cuts within the ["3.0", "5.0", "1.0"] kb ["BamHI", "EcoRI"] band
Homework Answers
The correct statement is as follows :
From there results, can be concluded that BamHI cuts within the 5.0 Kb EcoRI band.
Explanation :
After Digestion with EcoRI, three bands are obtained with length of 1.0 Kb, 3.0 Kb and 5.0 Kb.
But when we do double digestion with EcoRI + BamHI, four bands are obtained with length of 1.0 Kb, 1.5 Kb, 3 Kb and 3.5 Kb.
Now if we compare both the results, we can easily conclude that 1.0 Kb and 3.0 Kb bands are identical. Also, upon closer inspection it is found that the two new bands of 3.5 Kb and 1.5 Kb add up to form 5.0 Kb. So it means that BamHI actually digests the 5.0 Kb fragment of (EcoRI digest) into two fragments of 3.5 Kb and 1.5 Kb.
This situation can be easily depicted with a simple diagram for even better explanation :
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please select answer from options given in [] and are bold,
there are 3 parts
1....
An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products...
An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1, 3 and 5 kb are seen. How many EcoRI sites are in the plasmid? Choose the one answer that is most correct. Group of answer choices: At least 2, At least 3, At least 5, None, At least 1, At least 4
The PCR was a success and your target region of 770 bp in length has been...
The PCR was a success and your target region of 770 bp in length has been amplified. You now plan to digest the DNA amplicon with the restriction enzyme Eael, and clone the resulting longest fragment it into the Eael site of the 5 kb plasmid diagrammed below. 770 bp BamHI 1 200 EcoRI 800 EcoRI 4000 1000 5 kb O /1000 2000 2000 Faal You purify your recombinant plasmid from bacterial cells, and run the plasmid (uncut. or not...
You have determined that a bacterial strain you are working with contains a single type of...
You have determined that a bacterial strain you are working with
contains a single type of plasmid. You isolate the plasmid DNA and
digest separate portions of it with each of two different
restriction enzymes, BamHI and HpaI, and also perform a double
digest using both enzymes. You then fractionate the enzyme digests
on an agarose gel and stain the gel with ethidium bromideto
visualize the restriction fragment patterns. Your results are shown
below. Size markers (in nucleotide base pairs)...
UuOU Wuuur 3. Complete the table below to show your anticipated results, when digesting the recombinant...
UuOU Wuuur 3. Complete the table below to show your anticipated results, when digesting the recombinant plasmid containing the PCR fragment with the different restriction enzymes as listed. Recombinant plasmid Restriction enzyme No. of cuts Fragment sizes EcoRI BamHI Eael EcoRI + BamHI +Eae 1 -teamHI EcoRI 4000 EcoRI 6k6 1000 2000 es Target region of 770 bp in length has been amplified Plan to digest the DNA amplican with restrictch enzyme Egel, and clone the resulting longest fragment into...
please help if you can 3. You cloned a 20 kb piece of DNA, which contains...
please help if you can
3. You cloned a 20 kb piece of DNA, which contains restriction sites as shown below. 281534 5 B 4 & 5 B = BamHl site, E = EcoRI site, H = Hindill site Numbers above the segments represent the sizes of the regions in kb. Draw and label (in the agarose gel below) the sizes of the fragments you would expect to see after complete digestion of this piece of DNA with the following...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Can someone please help me answer these? Neat work is greatly appreciated. Explanation will also be...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
A linear piece of DNA has the following restrictions sites: You decided to set up an...
A linear piece of DNA has the following restrictions sites:
You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
a) Which restriction...
You have accidentally torn the labels off two tubes, each containing a different plasmid, and now...
You have accidentally torn the labels off two tubes, each
containing a different plasmid, and now do not know which plasmid
is in which tube. Fortunately, you have restriction maps for both
plasmids, shown in the figure below. You have the opportunity to
test just one sample from one of your tubes. You have equipment for
agarose-gel electrophoresis, a standard set of DNA size markers,
and the necessary restriction enzymes.
A)Why won’t making a single cut (e.g. with HindIII) help...
The PCR was a success and your target region of 770 bp in length has been amplified. You now plan to digest the DNA amplicon with the restriction enzyme Eael, and clone the resulting longest fragment it into the Eael site of the 5 kb plasmid diagrammed below. 770 bp BamHI 1 200 EcoRI 800 EcoRI 4000 1000 5 kb O /1000 2000 2000 Faal You purify your recombinant plasmid from bacterial cells, and run the plasmid (uncut. or not...
You have determined that a bacterial strain you are working with
contains a single type of plasmid. You isolate the plasmid DNA and
digest separate portions of it with each of two different
restriction enzymes, BamHI and HpaI, and also perform a double
digest using both enzymes. You then fractionate the enzyme digests
on an agarose gel and stain the gel with ethidium bromideto
visualize the restriction fragment patterns. Your results are shown
below. Size markers (in nucleotide base pairs)...
UuOU Wuuur 3. Complete the table below to show your anticipated results, when digesting the recombinant plasmid containing the PCR fragment with the different restriction enzymes as listed. Recombinant plasmid Restriction enzyme No. of cuts Fragment sizes EcoRI BamHI Eael EcoRI + BamHI +Eae 1 -teamHI EcoRI 4000 EcoRI 6k6 1000 2000 es Target region of 770 bp in length has been amplified Plan to digest the DNA amplican with restrictch enzyme Egel, and clone the resulting longest fragment into...
please help if you can
3. You cloned a 20 kb piece of DNA, which contains restriction sites as shown below. 281534 5 B 4 & 5 B = BamHl site, E = EcoRI site, H = Hindill site Numbers above the segments represent the sizes of the regions in kb. Draw and label (in the agarose gel below) the sizes of the fragments you would expect to see after complete digestion of this piece of DNA with the following...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
A linear piece of DNA has the following restrictions sites:
You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
a) Which restriction...
You have accidentally torn the labels off two tubes, each
containing a different plasmid, and now do not know which plasmid
is in which tube. Fortunately, you have restriction maps for both
plasmids, shown in the figure below. You have the opportunity to
test just one sample from one of your tubes. You have equipment for
agarose-gel electrophoresis, a standard set of DNA size markers,
and the necessary restriction enzymes.
A)Why won’t making a single cut (e.g. with HindIII) help...
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