Question

You have accidentally torn the labels off two tubes, each containing a different plasmid, and now do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in the figure below. You have the opportunity to test just one sample from one of your tubes. You have equipment for agarose-gel electrophoresis, a standard set of DNA size markers, and the necessary restriction enzymes.

A)Why won’t making a single cut (e.g. with HindIII) help you determine which plasmid is in your tube?

B)Why won’t cutting with all 4 enzymes at the same time help you determine which plasmid is in your tube?

BamHI BamHI Bglll 0.5 kb EcoRI 0.3 kb 0.2 k 0.4 kb 0.2 kb 1.0 kb 0.3kb EcoRI 1.0 kb Bgll 0.5 kb plasmid A plasmid B BamHI BamHl 0.4 kb Hindlll HindIII 1.0 kb EcoRI 1.0 kb CoRI PlasmidA Plasmid B

Answer 1

Same sizes B) planus fal te HindIll on both plamd prnt 1) Hindlll at Siw sike Hindul 4:厂| Sand - --| 3-4 kb. Singhemd 2-5 Rasalt: OblaǐhodSo will /

B) Vou ill hot two pl clan cnamn, you will ge, Same 3) twe o-5 kb 0-4 kb 03 kb en

Tf you aut ith Hindl and By thun you will Sin tuws planni k plamid A A) 3kb 2-

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