Question

You have determined that a bacterial strain you are working with contains a single type of plasmid. You isolate the plasmid DNA and digest separate portions of it with each of two different restriction enzymes, BamHI and HpaI, and also perform a double digest using both enzymes. You then fractionate the enzyme digests on an agarose gel and stain the gel with ethidium bromideto visualize the restriction fragment patterns. Your results are shown below. Size markers (in nucleotide base pairs) are indicated at the left side of the gel. Using these data, construct a possiblerestriction map for the plasmid

You have determined that a bacterial strain you are working with contains a single type of plasmid. You isolate the plasmid DNA and digest separate portions of it with each of two different restriction enzymes, BamHI and Hpal, and also perform a double digest using both enzymes. You then fractionate the enzyme digests on an agarose gel and stain the gel with ethidium bromide to visualize the restriction fragment patterns. Your results are shown below. Size markers (in nucleotide base pairs) are indicated at the left side of the gel. Using these data, construct a possible restriction map for the plasmid BamHI BamHI Hpal Hpal 2,100 1,500 900 800 ||| 700 400 200 -

Answer 1

BamH1 BamH1 Hpal Hpal 200 800 900 800 900 1500 2100 Hpal Hpal BamH1 700 BamH1 o 400 700 Hpal Hpal Digestion with Hpal Digestion with BamH1 Double digestion