Homework Answers
Since , Eco R1 cut at site creating a 5kb fragment , it must have cut the segment in two halves ( original length is 10kb ).
BamH1 has cut at two sites creating a 6kb , 3 kB and 1 kB
fragment .
Add Answer to:
Module 2 homework Laurel Jacqmai 0341 - Spring 20-MEEK > Activities and Due Dates > Module...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5 > A B C D E Restriction mapping of a linear piece of DNA reveals the EcoRI restriction sites. EcoRI site ! EcoRI site 2 2 kb. 4 kb 5 kb 11 kb 6 kb 5 kb 4 kb --3.5 kb 2 kb In each of the scenarios described, DNA is cut by EcoRI and the resulting fragments are separated by gel electrophoresis. The...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Can someone please help me answer these? Neat work is greatly appreciated. Explanation will also be...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
please help if you can 3. You cloned a 20 kb piece of DNA, which contains...
please help if you can
3. You cloned a 20 kb piece of DNA, which contains restriction sites as shown below. 281534 5 B 4 & 5 B = BamHl site, E = EcoRI site, H = Hindill site Numbers above the segments represent the sizes of the regions in kb. Draw and label (in the agarose gel below) the sizes of the fragments you would expect to see after complete digestion of this piece of DNA with the following...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
2- Spring 20 - EPSTEIN > Activities and Due Dates > Homework #5 - non-vascular plants...
2- Spring 20 - EPSTEIN > Activities and Due Dates > Homework #5 - non-vascular plants & ferns gnment Score: 20% Resource tion 5 of 20 ) Which plants are distinct clades of bryophytes? Club mosses liverworts gingkos horsetails mosses
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction...
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction enzymes. EcoRI produces fragments of sizes 2.9, 4.5. 6.2.7.4, and 8.0 kb. An autoradiogram developed from a Southern blot of this digest shows radioactivity associated with the 6.2 and 8.0 fragments. BamHI cleaves the same molecule into fragments of sizes 6.0, 10.1 and 12.9; the label is found to be associated with the 6.0 and 10.1 fragments. When EcoRI and BamHI are used together,...
L= No restriction B=Bam HI E=EcoRI H=Hind III Band 1 27mm 31mm 29mm 29mm Band 2...
L= No restriction
B=Bam HI
E=EcoRI
H=Hind III
Band 1
27mm
31mm
29mm
29mm
Band 2
NA
34mm
41mm
37mm
Band 3
NA
41mm
46mm
43mm
Band 4
NA
43mm
49mm
52mm
Band 5
NA
46mm
57mm
71mm
Band 6
NA
NA
NA
76mm
Above is the actual measurements for the distance in mm. Please
plug this in with the existing chart located above
Gel Electrophoresis lab assignment The following sheets will be used to demonstrate your knowledge of gel...
Assignment 19 Myah Boelk - 2- Spring 20 - SANDERS > Activities and Due Dates) Assignments...
Assignment 19 Myah Boelk - 2- Spring 20 - SANDERS > Activities and Due Dates) Assignments Assignment Score: 43.9% Resources Hint Check Answer Question 7 of 11 > For a particular reaction, AH = -28.4 kJ and AS = -87.9 J/K. Assuming these values change very little with temperature, at what temperature does the reaction change from nonspontaneous to spontaneous? Is the reaction in the forward direction spontaneous at temperatures greater than or less than the calculated temperature? O greater...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5 > A B C D E Restriction mapping of a linear piece of DNA reveals the EcoRI restriction sites. EcoRI site ! EcoRI site 2 2 kb. 4 kb 5 kb 11 kb 6 kb 5 kb 4 kb --3.5 kb 2 kb In each of the scenarios described, DNA is cut by EcoRI and the resulting fragments are separated by gel electrophoresis. The...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
please help if you can
3. You cloned a 20 kb piece of DNA, which contains restriction sites as shown below. 281534 5 B 4 & 5 B = BamHl site, E = EcoRI site, H = Hindill site Numbers above the segments represent the sizes of the regions in kb. Draw and label (in the agarose gel below) the sizes of the fragments you would expect to see after complete digestion of this piece of DNA with the following...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
2- Spring 20 - EPSTEIN > Activities and Due Dates > Homework #5 - non-vascular plants & ferns gnment Score: 20% Resource tion 5 of 20 ) Which plants are distinct clades of bryophytes? Club mosses liverworts gingkos horsetails mosses
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction enzymes. EcoRI produces fragments of sizes 2.9, 4.5. 6.2.7.4, and 8.0 kb. An autoradiogram developed from a Southern blot of this digest shows radioactivity associated with the 6.2 and 8.0 fragments. BamHI cleaves the same molecule into fragments of sizes 6.0, 10.1 and 12.9; the label is found to be associated with the 6.0 and 10.1 fragments. When EcoRI and BamHI are used together,...
L= No restriction
B=Bam HI
E=EcoRI
H=Hind III
Band 1
27mm
31mm
29mm
29mm
Band 2
NA
34mm
41mm
37mm
Band 3
NA
41mm
46mm
43mm
Band 4
NA
43mm
49mm
52mm
Band 5
NA
46mm
57mm
71mm
Band 6
NA
NA
NA
76mm
Above is the actual measurements for the distance in mm. Please
plug this in with the existing chart located above
Gel Electrophoresis lab assignment The following sheets will be used to demonstrate your knowledge of gel...
Assignment 19 Myah Boelk - 2- Spring 20 - SANDERS > Activities and Due Dates) Assignments Assignment Score: 43.9% Resources Hint Check Answer Question 7 of 11 > For a particular reaction, AH = -28.4 kJ and AS = -87.9 J/K. Assuming these values change very little with temperature, at what temperature does the reaction change from nonspontaneous to spontaneous? Is the reaction in the forward direction spontaneous at temperatures greater than or less than the calculated temperature? O greater...
Most questions answered within 3 hours.
-
Write a program to solve the Josephus problem, with the following
modification:
Sample Input:
./a.out n...
asked 55 minutes ago -
At the start of a CD it is spinning at a rate of 525 rpm
(revolutions...
asked 1 hour ago -
4. Without doing any calculations, predict whether the observed
∆T would increase, decrease or remain the...
asked 2 hours ago -
Based on the range, which of the following sets of scores has
the greatest variability? 3,...
asked 3 hours ago -
Ripples in a pond travel at a velocity of 3 m/s with one peak
passing a...
asked 3 hours ago -
A man stands on the roof of a building of height 13.0 mm and
throws a...
asked 3 hours ago -
The extent to which assets are financed by borrowed funds and
other liabilities is indicated by:...
asked 4 hours ago -
Explain in detail
Germany is the fifth largest economy
explain what goods and services Germany specializes...
asked 5 hours ago -
The density of platinum is 21.45 g/mL. If a cube of platinum
with a mass of...
asked 5 hours ago -
Accounts Receivable
Sales
A/R Posting
Extended Sales Invoice
Packing Slip
Compare invoice to packing slip 2...
asked 5 hours ago -
Michaella, age 23, is a full-time law student and is claimed by
her parents as a...
asked 5 hours ago -
Why are polymers not typically casted into products?
asked 5 hours ago