Question

Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5 > A B C D E Restriction mapping of a linear piece of DNA reveals the EcoRI restriction sites. EcoRI site ! EcoRI site 2 2 kb. 4 kb 5 kb 11 kb 6 kb 5 kb 4 kb --3.5 kb 2 kb In each of the scenarios described, DNA is cut by EcoRI and the resulting fragments are separated by gel electrophoresis. The gel image is pictured Match each of the scenarios to the lane on the gel that would result after digestion and gel electrophoresis, Digestion is performed on the linear DNA, as shown A mutation that alters EcoRI site I occurs in this piece of DNA Answer Bank Mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA. 1000 bp of DNA are inserted between the two restriction sites. 500 bp of DNA between the two restriction sites are deleted. Polisher WH Freeman Question Source: Mercebe Genes A Conceptual Approach

Answer 1

Answer:

• Digestion performed on the linear DNA would result in the generation of 2 kb, 4 kb, and 5 kb DNA fragments.
• Digestion of linear DNA by EcoRI at site 1 will generate a 2 kb DNA fragment and a 9 kb DNA fragment.
• Further digestion of DNA with EcoRI at site 2 will fragment the 9 kb DNA into 4 kb and 5 kb DNA fragments.
• So, lane C represents the scenario that would result after digestion is performed on linear DNA.

2):

• If there is mutation in DNA that alters the EcoRI site 1, then EcoRI cannot fragment the DNA at site 1.
• However EcoRI fragments the DNA at site 2 generating 6 kb and 5 kb DNA fragments.
• So, lane A represents the scenario that would result after digestion is performed on DNA in which a mutation has altered the EcoRI site 1.

3):

• If there are mutations that alter both EcoRI site 1 and EcoRI site 2, then the enzyme cannot recognize the restriction sites and fragment the DNA at both the sites.
• Thus, DNA is not fragmented and remains in a linear form of 11 kb size.
• So, lane B represents the scenario that would result after digestion is performed on DNA in which mutations have altered both EcoRI site 1 and site 2.

4):

• If 1000 bp DNA is inserted between the two restricted sites, then the size of DNA between the two restricted sites becomes 5 kb in size.
• Digestion of this DNA by EcoRI at site 1 and site 2 will result in 2 kb, 5 kb and 5 kb fragments.
• The 5 kb fragment DNA band intensity is more in lane E due to the two 5 kb DNA fragments that are generated after digestion of 1000 bp inserted DNA.
• So, lane E represents the scenario that would result after digestion is performed on DNA in which 1000 bp of DNA is inserted between the two restriction sites.

5):

• If 500 bp DNA is inserted between the two restricted sites, then the size of DNA between the two restricted sites becomes 3.5 kb in size.
• Digestion of this DNA by EcoRI at site 1 and site 2 will result in 2 kb, 3.5 kb and 5 kb fragments.
• Lane D has a 3.5 kb fragment.
• So, lane D represents the scenario that would result after digestion is performed on DNA in which 500 bp of DNA is deleted between the two restriction sites.

Please Rate My Answer..... Thank....u...