Homework Answers
a)
|
ENZYME |
PRODUCT |
TOTAL |
|
Afe1 |
5000 bp |
5000 bp |
|
Sma1 |
2900 bp & 2100 bp |
5000 bp |
|
Pst1 |
5000 bp |
5000 bp |
Hence the plasmid is 5000 bp large.
b) Restriction Map
- Consider the digestion of Afe1 and Pst1. These are generating two fragments of 4000 bp and 1000 bp
- Then consider the mixed digestion.
- The final map:-
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