Question

1. ) A portion of a specific DNA molecule consists of the following sequence of nucleotides:

TAC GAA CTT CGG TCC ATT

This DNA sequence codes for the following short polypeptide:

methionine – leucine – glutamic acid – proline – arginine

a) Describe the steps in the synthesis of this polypeptide, starting with the synthesis of mRNA.

2.) 

Demonstrate dehydration synthesis through the bonding of two monomers of a

macromolecule. Feel free to use carbohydrates, lipids, or amino acids. Show the starting material and what it looks like afterwards, and don’t forget other byproduct(s). What kind of bond has formed here?

a ) Select two factors that influence enzyme activity and explain how.

3.) 

In order to create a DNA fingerprint, a segment of DNA must be amplified using the polymerase chain reaction (PCR). Briefly outline the steps in PCR.

a ) Explain TWO applications of DNA fingerprinting.

4.)

Technological tracking has indicated that there are 480 moose living in a 600 hectare section of Algonquin Park. This section of the park contains 4 lakes that are approximately 12 hectares each. What is the ecological density of the moose?