Homework Answers
Add Answer to:
ou are given absorption data of a DNA and a protein solution. Draw an absorption spectrum...
Q3. (10 points) The following shows the absorption spectrum of aqueous solution of V2. Based on c...
Q3. (10 points) The following shows the absorption spectrum of aqueous solution of V2. Based on complementary color's principle (and emphasizing only at visible range of the spectrum), what is the color of the solution? Explain your answer based on the provided spectrum (mainly for 0.1 M) 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.1M 0.02M 200 300 400 500 600 700 800 Wavelength (nm)
Q3. (10 points) The following shows the absorption spectrum of aqueous solution of...
After purification of your favorite plasmid you use a spectrophotometer to find the concentration of your...
After purification of your favorite plasmid you use a
spectrophotometer to find the concentration of your DNA and observe
the graph below. Resketch by hand this graph on your answer page
with exact axis labels and scales. Redraw the red line. Draw, in
your estimation, the absorption spectrum of what is expected of a
good DNA prep.
OD o X Load your sample and press the measure button. Blank Re-Blank Sample ID: Pedestal ort pectra ple volume Type: DNA 50.00...
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain...
Q5: (2) DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values listed below. The DNA had been diluted 10 fold. Remember that a solution of DNA that is 50μg/ml (or 50ng/μl) has an absorbance of 1.0 at a wavelength of 260 nm. What is the concentration of the DNA in this sample (In this question DO NOT ignore the problem of dust in the sample and give answer in ng/ul). A260 = 0.80...
Identify the structure. TTITITTTTTTT M 2270 problem 5 IR spectrum (liquid film) 1745 4000 3000 2000...
Identify the structure.
TTITITTTTTTT M 2270 problem 5 IR spectrum (liquid film) 1745 4000 3000 2000 V 03 1600 ) 12008 (cm mass spectrum yo absorbance UV spectrum no significant absorption above 210nm og 86 Mt 113 CH,NO 250- 300 (nm) TTT 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 310 m/e TT ZOMHz carbon-13 NMR spectrum CDCi, solution off-resonance decoupled proton decoupled 220 - 200 180 160 140 120 100 80...
Questions 1. Consider the calibration graph and the absorption spectrum for solutions of metal ion, M...
Questions 1. Consider the calibration graph and the absorption spectrum for solutions of metal ion, M * Absorption Spectrum for M Calibration Curve for M 0.8 0.7 1.2 0.6 1 0.5 S 0.8 0.4 0.6 0.3 0.4 0.2 0.1 0.2 0- 0 0.5 0.4 0.3 0.2 0.1 400 450 500 550 600 650 700 [M*1 (Molar) (入) nm a) At what wavelength was the calibration curve obtained? Explain your answer. b) Use the calibration curve to calculate the concentration (M)...
LLI (nM) 92 The data at right describe the binding of a ligand to a protein:...
LLI (nM) 92 The data at right describe the binding of a ligand to a protein: 0.1 0.07 s [L] is the concentration of free ligand. e is the fraction of sites on the protein that are occupied by the ligand. (Note that some textbooks use terms like v or Y, instead of 6, to denote fractional saturation) 0.4 0.23 0.36 Answer parts (a) and (b) below. 0.55 1.2 (a) Which of the following graphs could be used to estimate...
1. (20 pts) Reduction of the disulfide bond in human PrP91-231 (prion protein) and lowering the pH to 4.0, in a dilute...
1. (20 pts) Reduction of the disulfide bond in human PrP91-231 (prion protein) and lowering the pH to 4.0, in a dilute acetate buffer generated a highly soluble monomeric protein. Figure 1. Determination of the apparent molecular weight of PrP by size-exclusion chromatography. (A) Elution profile of standards used to construct a calibration curve of molecular weight (MW) versus elution time. (B) Oxidized human PrP (native form) (pH 8.0) elutes with an apparent MW of 18 kD. (C) Reduced human...
IR Spectra help, question up top Use IR. proton NMR and MS data to deduce the...
IR Spectra help, question up top
Use IR. proton NMR and MS data to deduce the correct structure Draw the structure and dide it. Indicate on the IR, NMR and MS spectra the information you got from each to deduce the structure. Problem 20 IR Spectrum 4000 3000 2000 1800 12000 00 8 Mass Spectrum 8 8 No significant UV absorption above 220 nm 8 8 CgH NO 240 280 40 80 120 180 200 13C NMR Spectrum (1000 MHE....
Problem 115: Determine the molecule of C7H12O The IR, mass spec, CNMR and HNMR needs to...
Problem 115: Determine the molecule of
C7H12O
The IR, mass spec, CNMR and HNMR needs to be typed out not
handwritten, and the molecule needs to be a digital
representation as well. A base example is provided below:
________________________________________________________________________________________________________
Find and draw the molecule based on these charts (Drawing needs
to be digital and please type out answer)
Use an analysis of each graph and explain what numbers indicate
what groups.
Base Example
IR:
3500: O-H group
1750: Ester
Mass...
Find the compounds using IR and NMR: Problem 25 2251 IR Spectrum (liquid film) 800 1600...
Find the compounds using IR and NMR:
Problem 25 2251 IR Spectrum (liquid film) 800 1600 1200 4000 3000 2000 v (cm) 100 Mass Spectrum M 117 80 77 60 91 40 20 CgH7N 200 80 120 160 240 280 40 m/e expansion 13C NMR Spectrum (100.0 MHz, CDCI, solution) 130 129 ppm DEPT CH Cн,! сн! expansion 130 129 ppm solvent proton decoupled (ppm) 40 200 160 120 80 H NMR Spectrum (400 MHz. CDCI, solution) TMS 10 7...
Q3. (10 points) The following shows the absorption spectrum of aqueous solution of V2. Based on complementary color's principle (and emphasizing only at visible range of the spectrum), what is the color of the solution? Explain your answer based on the provided spectrum (mainly for 0.1 M) 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.1M 0.02M 200 300 400 500 600 700 800 Wavelength (nm)
Q3. (10 points) The following shows the absorption spectrum of aqueous solution of...
After purification of your favorite plasmid you use a
spectrophotometer to find the concentration of your DNA and observe
the graph below. Resketch by hand this graph on your answer page
with exact axis labels and scales. Redraw the red line. Draw, in
your estimation, the absorption spectrum of what is expected of a
good DNA prep.
OD o X Load your sample and press the measure button. Blank Re-Blank Sample ID: Pedestal ort pectra ple volume Type: DNA 50.00...
Identify the structure.
TTITITTTTTTT M 2270 problem 5 IR spectrum (liquid film) 1745 4000 3000 2000 V 03 1600 ) 12008 (cm mass spectrum yo absorbance UV spectrum no significant absorption above 210nm og 86 Mt 113 CH,NO 250- 300 (nm) TTT 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 310 m/e TT ZOMHz carbon-13 NMR spectrum CDCi, solution off-resonance decoupled proton decoupled 220 - 200 180 160 140 120 100 80...
Questions 1. Consider the calibration graph and the absorption spectrum for solutions of metal ion, M * Absorption Spectrum for M Calibration Curve for M 0.8 0.7 1.2 0.6 1 0.5 S 0.8 0.4 0.6 0.3 0.4 0.2 0.1 0.2 0- 0 0.5 0.4 0.3 0.2 0.1 400 450 500 550 600 650 700 [M*1 (Molar) (入) nm a) At what wavelength was the calibration curve obtained? Explain your answer. b) Use the calibration curve to calculate the concentration (M)...
LLI (nM) 92 The data at right describe the binding of a ligand to a protein: 0.1 0.07 s [L] is the concentration of free ligand. e is the fraction of sites on the protein that are occupied by the ligand. (Note that some textbooks use terms like v or Y, instead of 6, to denote fractional saturation) 0.4 0.23 0.36 Answer parts (a) and (b) below. 0.55 1.2 (a) Which of the following graphs could be used to estimate...
1. (20 pts) Reduction of the disulfide bond in human PrP91-231 (prion protein) and lowering the pH to 4.0, in a dilute acetate buffer generated a highly soluble monomeric protein. Figure 1. Determination of the apparent molecular weight of PrP by size-exclusion chromatography. (A) Elution profile of standards used to construct a calibration curve of molecular weight (MW) versus elution time. (B) Oxidized human PrP (native form) (pH 8.0) elutes with an apparent MW of 18 kD. (C) Reduced human...
IR Spectra help, question up top
Use IR. proton NMR and MS data to deduce the correct structure Draw the structure and dide it. Indicate on the IR, NMR and MS spectra the information you got from each to deduce the structure. Problem 20 IR Spectrum 4000 3000 2000 1800 12000 00 8 Mass Spectrum 8 8 No significant UV absorption above 220 nm 8 8 CgH NO 240 280 40 80 120 180 200 13C NMR Spectrum (1000 MHE....
Problem 115: Determine the molecule of
C7H12O
The IR, mass spec, CNMR and HNMR needs to be typed out not
handwritten, and the molecule needs to be a digital
representation as well. A base example is provided below:
________________________________________________________________________________________________________
Find and draw the molecule based on these charts (Drawing needs
to be digital and please type out answer)
Use an analysis of each graph and explain what numbers indicate
what groups.
Base Example
IR:
3500: O-H group
1750: Ester
Mass...
Find the compounds using IR and NMR:
Problem 25 2251 IR Spectrum (liquid film) 800 1600 1200 4000 3000 2000 v (cm) 100 Mass Spectrum M 117 80 77 60 91 40 20 CgH7N 200 80 120 160 240 280 40 m/e expansion 13C NMR Spectrum (100.0 MHz, CDCI, solution) 130 129 ppm DEPT CH Cн,! сн! expansion 130 129 ppm solvent proton decoupled (ppm) 40 200 160 120 80 H NMR Spectrum (400 MHz. CDCI, solution) TMS 10 7...
Most questions answered within 3 hours.
-
find the critical value associated with a right sided hypothesis of
the mean using a sample...
asked 51 minutes ago -
Which of the following statements is true with respect to a
simple linear regression model?
a....
asked 1 hour ago -
The energy required to remove an electron from a surface of a
solid element is called...
asked 2 hours ago -
How would you train your employees to avoid risks of using
mobile devices?
asked 3 hours ago -
Assume Kw = 1.01 ✕ 10−14
For pure water, we can calculate [H3O+ ] = [OH...
asked 4 hours ago -
Suppose that on a temperature scale X, water boils at 203.0°X
and freezes at -105.7°X. What...
asked 5 hours ago -
BaS crystallizes in a cubic unit cell with S2- ions on each
corner and each face....
asked 5 hours ago -
A. 0≤P(Oi)≤10≤P(Oi)≤1 for each i
B. P(Oi)≤0P(Oi)≤0
C. P(Oi)=1+P(OCi)P(Oi)=1+P(OiC)
D. P(Oi)≥1P(Oi)≥1
If an experiment consists of...
asked 7 hours ago -
A battery has an emf of 9.20V and an internal resistance of 1.20
ohm. a)What resistance...
asked 7 hours ago -
The area of an elastic circular loop decreases at a constant
rate, dA/dt = −6.60×10−3 m2/s...
asked 8 hours ago -
The denaturation of proteins can be described by the
equilibrium
F⇌U
where F and U represent...
asked 9 hours ago -
Please answer what the maximum and minimum force is, and the
angle on the ion is...
asked 9 hours ago