can we use gas chromatography to seperate protein mixture and explain
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To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pKa is 6.0). If I wanted to seperate protein X, whose pl is 3.0x what is the pH range of the buffer that can be used for ion exchange chromatography with this resin ? A. any pH between 3.0 and 6.0 B. there is no pH range for this resin and protein X C. any pH above 4.0 D. any pH below 6.0
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
How would you use ion exchange chromatography to seperate a mixture of alanine, glutamic acid, and lysine if you wished to maintain the pH at 7.5 and use only a single ion exchange column ( either an anionic or cationic exchanger). Describe the expected order of elution under the conditions which you propose.
Explain the various factors responsible for separation of components of a mixture in chromatography. Explain about the classification of chromatography with few examples. Draw a block diagram of gas chromatograph and explain the function of each component Write a note on the micro syringe use to inject the liquid sample into gas chromatograph. Write a note on principle of working of Gas chromatograph. (How to prepare GC for analysis) Write a note on analysis of chromatogram (Types the method of...
In gas chromatography (GC), why do we use a FAME standard instead of an alkane standard and how can we still use this to calculate a retention index?
1. Robin is planning to use AKTA system chromatography with SP HiTrap column. She will use a linear NaCI gradient to separate her protein mixture at pH 6.5. The protein mixture contains: fibrinogen (pl 5.8), hemoglobin (pl 7.1), ribonuclease A (pl 7.8), lysozyme (pl 11) and pepsin (pl 1). a) What type of resin is SP HiTrap? (1 point) b) Indicate which proteins (of any) will not bind to the column. Then, predict the order in which the proteins will...
Lab Day 8: Chromatography Prelab Questions 1) The basic food colors you can buy are red, blue areen, and yellow. Think about what you know about colors and predict which of these might be a single colored compound and which ones might contain more than one. write down your prediction. If you have the colors listed and want a purple color, what would you do? 2) Put the following chromatography solvents in order of polarity as a chromatography solvent: acetone,...
Combined chromatography methods You are provided with a solution containing a mixture of three different proteins with the following characteristics. Protein mol. mass (Da) isoelectric point (pI) Binds to ligand? A 45,000 6.1 Yes B 84,000 7.8 No C 85,000 7.9 Yes D 120,000 6 No E 122,000 8.0 No You are also provided with a size exclusion column, an ion-exchange column, and an affinity purification column (containing the ligand recognised by proteins A and C above). With the information...
1. you will be doing chromatography with a mixture of water and n-propanol. would it make sense to add hexane to this mixture to try and make it work better? EXPLAIN 2. Two cars full of people took a trip today. car A had a lot of kids and stopped at lots of rest stops and gas stations to give them a break. Car B had just adults in it, and only stopped once. Of course Car B got to...
Ion Exchange Chromatography Experiment 1. What is the basis for the seperation of different compounds by ion exchange? 2. How can molecules with the same charge at varying amounts be seperated by chromatography? 3. Why are celluloses often used as supports to seperate large biologically active proteins? 4. Why is it important to prepare a standard curve for each spectrophotometer? 5. What would happen if 0.5M K+ acetate were used to elute the sample? Why? (0.01M KOAc was actually used)