In gas chromatography (GC), why do we use a FAME standard instead of an alkane standard...
In gas chromatography (GC), why do we use a FAME standard instead of an alkane standard and how can we still use this to calculate a retention index?
Homework Answers
If you use FAME polyethylene glycol column, 30 meters or 100 meters with temperature ramping starting from 50 oC to 225 oC with 3 oC increase per minute. Phenolic groups can be detected but not on the FAME column, but RTX5 should do the job for that using GC-MS.
Nitrogen content will be best estimated using the Kjeldahl apparatus.
The main advantage of FAME standard over alkane standard is that FAME is more polar than the alkane.
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In gas chromatography (GC), why do we use a FAME standard
instead of an alkane standard...
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For the three gas chromatography graphs in the picture, how do I calculate the ratio of...
For the three gas chromatography graphs in the picture, how do I
calculate the ratio of Cl:Br?
I believe it has something to do with the area of the peaks (given
in the tables below the graphs).
Also, how do I determine which is a better nucleophile and
why?
Thank you!
Use this table to correlate the peaks in the GC printouts to product identification. Then use the table entries for area or area% in the GC results to calculate...
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chromatography mechanisms
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Lab #2: Gas Chromatography Theory behind gas chromatography What do all types of chromatography share in common? Relationship between retention time and boiling point/vapor pressure How changing parameters affect the retention time Ramp rate Temperature Length of column Pressure Whether column is packed or unpacked
For the three gas chromatography graphs in the picture, how do I
calculate the ratio of Cl:Br?
I believe it has something to do with the area of the peaks (given
in the tables below the graphs).
Also, how do I determine which is a better nucleophile and
why?
Thank you!
Use this table to correlate the peaks in the GC printouts to product identification. Then use the table entries for area or area% in the GC results to calculate...
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent ion= 2.05Smin low Peak 1 Compound Name: athanol Rapesnal utanal aclapeatanal 4.755min oilng 6.895min .230 min Peak 2 Compound Name: Peak 3 Compound Name: Peak 4 Compound Name: GC#:1 Ketone Standard Mixture Retention time 2.575un Peak 1 Compound Name: Acclono Butanon a Pentanone 2tHex anon Peak 2 Compound Name: min Peak 3 Compound Name: 615 min 1D. 307 min Peak 4 Compound Name: Part...
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
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