Question

Which of the following is not needed to properly perform aseptic technique? A. Disinfecting the work area B. Sterilizing loops and needles before you take a sample of bacteria C. Sterilizing loops and needles after you inoculate media OD. Wearing latex gloves E. Flaming the culture tube after you open it and before you close it

Answer 1

Ans) The correct answer is "D" i.e wearing latex gloves (it is not needed to properly perform aseptic technique but it is still recommended.We mostly use ethyl alcohol 70-95% to wash our hands and then perform the aseptic procedures).

Option A is incorrect because disinfecting the work area is very important otherwise the micro organisms present in the surrounding area may contaminate the culture media or nutrient broth etc.

Option B and C are incorrect because sterilizing the loops and needles before and after picking up the sample of bacteria is important.If the loops and needles are not sterilized, it can result in cross-contamination.

Option E is incorrect because it is essential to pass the culture tube gently over the flame to sterilize the mouth of the culture tube so that no micro organisms will enter inside the culture tube.It should be noted that the culture should not touch the cotton plug or else it may get contaminated.