Homework Answers
Request Answer!
We need at least 10 more requests to produce the answer.
0 / 10 have requested this problem solution
The more requests, the faster the answer.
Add Answer to:
Please help! and show and explain. appreciate it!!
BamH1 3. The restriction map of a plasmid...
10) I was asked to perform double digestion of a given plasmid (map below) with restriction...
10) I was asked to perform double digestion of a given plasmid (map below) with restriction enzymes EcoRI and HindITI so that I can perform ligation of gene of interest into another vector cut by same enzymes. While setting up digestion reaction I was talking to my friend and mistakenly added EcoRV instead of EcoRI. Genere Please draw bands on gel (hypothetical sizes) L UC C LEDNA Ladder UC = Uncut Plasmid C = Cut Plasmid TIITTIIIII 1000
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes Smal, Bglil and Aval. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb Smal 5.9 4.3 BgII 8.2 2.0 Aval 5.3 4.9 Smal+ BglII 5.4 2.8 0.5 Smal+ Aval 3.3 2.6 2.3 2.0 Smal+-Bg!!! 5.3 2.1 2.0 0.8 (a) Draw an agarose gel with restriction fragments from the data...
A genetics problem covering chapter 10 concepts. Could really use some help! The Notch gene, involved...
A genetics problem covering chapter 10 concepts. Could
really use some help!
The Notch gene, involved in Drosophila development, is contained within a restriction fragment of Drosophila genomic DNA produced by cleavage with the enzyme SalI. The restriction map of this Drosophila fragment for several enzymes (Sall, PstI, and Xhol) is shown here; numbers indicate the distances between adjacent restriction sites. This fragment is cloned by sticky-end ligation into the single Sall site of a bacterial plasmid vector that is...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in Figure 5. Create a figure predicting the appearance of a gel showing digests with EcoRI, BamHI, and EcoRI and BamHI combined. Your figure should include a lane showing a 1kbladder for reference. (A photo of a 1kb ladder is provided in Figure 3A.) > > FOORIOL ULO BamHI (620) 325 BamHI (3090) Abel 23 Plasmid 4000bp BamHI (945) 2145 Figure 5. A restriction map...
please help me with these questions Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
10) I was asked to perform double digestion of a given plasmid (map below) with restriction enzymes EcoRI and HindITI so that I can perform ligation of gene of interest into another vector cut by same enzymes. While setting up digestion reaction I was talking to my friend and mistakenly added EcoRV instead of EcoRI. Genere Please draw bands on gel (hypothetical sizes) L UC C LEDNA Ladder UC = Uncut Plasmid C = Cut Plasmid TIITTIIIII 1000
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes Smal, Bglil and Aval. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb Smal 5.9 4.3 BgII 8.2 2.0 Aval 5.3 4.9 Smal+ BglII 5.4 2.8 0.5 Smal+ Aval 3.3 2.6 2.3 2.0 Smal+-Bg!!! 5.3 2.1 2.0 0.8 (a) Draw an agarose gel with restriction fragments from the data...
A genetics problem covering chapter 10 concepts. Could
really use some help!
The Notch gene, involved in Drosophila development, is contained within a restriction fragment of Drosophila genomic DNA produced by cleavage with the enzyme SalI. The restriction map of this Drosophila fragment for several enzymes (Sall, PstI, and Xhol) is shown here; numbers indicate the distances between adjacent restriction sites. This fragment is cloned by sticky-end ligation into the single Sall site of a bacterial plasmid vector that is...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in Figure 5. Create a figure predicting the appearance of a gel showing digests with EcoRI, BamHI, and EcoRI and BamHI combined. Your figure should include a lane showing a 1kbladder for reference. (A photo of a 1kb ladder is provided in Figure 3A.) > > FOORIOL ULO BamHI (620) 325 BamHI (3090) Abel 23 Plasmid 4000bp BamHI (945) 2145 Figure 5. A restriction map...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
Most questions answered within 3 hours.
-
Oxidative phosphorylation is achieved through chemiosmotic
coupling, which turns chemical energy into osmotic potential energy
that...
asked 1 hour ago -
Hollywood Shoes would like to maintain their cash account at a
minimum level of $64,000, but...
asked 1 hour ago -
Proteins CtrA-P, DnaA, and GcrA behave similarly to what
molecule in the Eukaryotic Cell Cycle?
asked 1 hour ago -
TRUE OR FALSE
In case of a competitive tender procedure, in the request for
quotation, the...
asked 1 hour ago -
I am a little behind please provide some explanation, will
provide thumbs up.
This question considers...
asked 1 hour ago -
Let say the utility function as U(X,Y) = lnX + Y. Show that the
marginal rate...
asked 1 hour ago -
A current of I=8.0A is flowing in a typical extension cord of
length L=3.00m. The cord...
asked 1 hour ago -
1. When balancing the equation H2SO4 (aq) + NaOH (aq) →
Na2SO4(aq) + H2O(aq), would you...
asked 2 hours ago -
Provide a definition of “classification hierarchy” and
illustrate your definition with an example of how and...
asked 2 hours ago -
1. Describe the four main green solvents. Provide one example of
the use of each solvent....
asked 2 hours ago -
International ethics focus on:
1) exploitation of labor
2)corruption
3) environmental impact
4) none of the...
asked 2 hours ago -
Costing Models You work for XYZ trucking and your boss has asked
you to figure out...
asked 2 hours ago