Question

Transgenic plants can be generated using T-DNA plasmid carrying a gene of interest. To get the DNA into the plant cells, the researchers

	a.	use a syringe needle to inject the DNA into pollen before fertilization
	b.	use a syringe needle to inject the DNA into groups of cells in the plant’s root tissue
	c.	co-cultivate bacteria that produce T-DNA along with plant cells, resulting in DNA transfer
	d.	add a mild detergent to cultures of plant cells to open holes in the cell wall.
		e. remove the plant cell’s normal DNA and replace it with the T-DNA.

Answer 1

The T-DNA plasmid or the Ti plasmid or the tumor inducing plasmid is the most common and natural method to induce recombination in plant cells. The T-plasmid or DNA is a natural transforming agent which does not require any chemical or physical facilitation for introducing a gene of interest in the host cell. Since it is naturally composed of transfection sequences, mere co-cultivation of target cells with T-DNA containing cells previously infused with gene of interest will result in transformation.

So, based upon above information, option c is most simple and correct.