Specifically, how does digestion with various restriction enzymes ensure the correct DNA had been isolated or...
Specifically, how does digestion with various restriction enzymes ensure the correct DNA had been isolated or amplified?
Homework Answers
ANSWER: Restriction endonuclease is a type of enzyme but it is having the speciality as it cleave only at specific restriction site which ensures the correct DNA to be isolated and amplified.
Explanation: Enzymes are having ability to catalyze reactions inside our body. In our body we have DNA, RNA sequences. The restriction endonuclease are the type of endonuclease ( means they cut the DNA sequence from middle by breaking the phosphodiester bond to form different copies) which cleave the DNA segment on the phosphodiester linkage but they are restricted for their work as there are sites( sequences/ nucleotides) which is firstly recognized by restriction endonuclease somit can break from the recognition site.
Restriction endonuclease cut the DNA at the unique sequence at non terminal region so as to generate the rejoinable DNA fragments. The recognition sites are generally of 4-8 base pairs. The genome of an organism have several restriction sites for one restriction enzyme.The distance between adjacent restriction site varies so they are generating the DNAfragments of different length but by its site specific character we can ensure the correct DNA had been isolated or amplified.
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Specifically, how does digestion with various restriction
enzymes ensure the correct DNA had been isolated or...
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated...
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated from bacteriophage lambda. This bacteriophage is a virus that infects E. coli and can undergo specialized transduction (see the transduction lab lecture). Assuming you obtained the following fragments from the restriction digestion. Draw the restriction map for the EcoRI and Hindi II enzymes in the 2. DNA. Fragment Size EcoRI 15300 6200 5250 2100 EcoRI + HindIII 15300 4400 4000 2100 1800 Hind!!! 17100...
Question 2: EcoRI, EcoRII, and EcoRV are all restriction enzymes found in E.coli. How does E.coli...
Question 2: EcoRI, EcoRII, and EcoRV are all restriction enzymes found in E.coli. How does E.coli prevent these enzymes from digesting its own DNA? Question 3: When performing a restriction digest it is necessary to incubate the enzymes and the DNA together to give the enzymes time to work. Given that we're using enzymes isolated from E.coli, a human symbiont, what temperature would you incubate the enzymes at, and why? Question 4: Why do DNA fragments migrate across the gel...
Restriction Enzymes are naturally found in bacteria that use them to A. Replicate DNA B. Correct...
Restriction Enzymes are naturally found in bacteria that use them to A. Replicate DNA B. Correct mutated DNA C. Cut up the DNA of invading Bacteriophage D. complete conjugation
Once the DNA has been collected from both a crime scene and suspect and amplified, what...
Once the DNA has been collected from both a crime scene and suspect and amplified, what are the samples treated with to make fragments of various sizes that can be loaded onto an agarose gel? O restriction enzymes heat DNAases
QUESTION 6 How was the identity of Kim's biological father determined? [2 pt] DNA restriction digestion...
QUESTION 6 How was the identity of Kim's biological father determined? [2 pt] DNA restriction digestion and gel electrophoresis Southern blot analysis Western blot analysis DNA sequencing
What are restriction enzymes and how do they affect DNA? Why do some fragments move quickly...
What are restriction enzymes and how do they affect DNA? Why do some fragments move quickly and some move slowly through an agarose gel? How can type II restriction enzymes and agarose gels be used to identify samples from individuals with the similar DNA sequence?
Question 18 4 pts What role do restriction enzymes play in bacteria? How do bacteria protect...
Question 18 4 pts What role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes? Change the surface proteins of bacteria; since DNA is not protein, there is no need for protection Cut foreign DNA into pieces; bacteria have RNA genomes. Destroy invading viral DNA: bacterial DNA does not contain the restriction enzyme recognition sequences. Restrict the growth rate of bacteria; bacterial DNA is restriction enzyme resistant. Question 18 4...
Day 1, You have isolated DNA from 30 individuals and performed restriction enzyme digestion. You have...
Day 1, You have isolated DNA from 30 individuals and performed
restriction enzyme digestion. You have loaded the product and run
the gel electrophoresis following the instruction properly. You
expected to see DNA bands after the procedure is completed, like
figure A, but after the completion of the run your gel looks like
figure B. You did not see any DNA band. You were so upset, but your
lab partner fixed the problem and your gel looked like figure A....
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
1. A small piece of DNA was digested using restriction enzymes HindIII and PstI: A) How...
1. A small piece of DNA was digested using restriction enzymes
HindIII and PstI:
A) How many basepairs long is the fragment?
a. 900 bp
b. 2000 bp
c. 600 bp
d. 500 bp
B) How many PstI restriction sites are located within
this fragment?
a. 0
b. 3
c. 1
d. 4
e. 2
Psti Pstl Hindili 500 500 100 900
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated from bacteriophage lambda. This bacteriophage is a virus that infects E. coli and can undergo specialized transduction (see the transduction lab lecture). Assuming you obtained the following fragments from the restriction digestion. Draw the restriction map for the EcoRI and Hindi II enzymes in the 2. DNA. Fragment Size EcoRI 15300 6200 5250 2100 EcoRI + HindIII 15300 4400 4000 2100 1800 Hind!!! 17100...
Question 2: EcoRI, EcoRII, and EcoRV are all restriction enzymes found in E.coli. How does E.coli prevent these enzymes from digesting its own DNA? Question 3: When performing a restriction digest it is necessary to incubate the enzymes and the DNA together to give the enzymes time to work. Given that we're using enzymes isolated from E.coli, a human symbiont, what temperature would you incubate the enzymes at, and why? Question 4: Why do DNA fragments migrate across the gel...
Once the DNA has been collected from both a crime scene and suspect and amplified, what are the samples treated with to make fragments of various sizes that can be loaded onto an agarose gel? O restriction enzymes heat DNAases
QUESTION 6 How was the identity of Kim's biological father determined? [2 pt] DNA restriction digestion and gel electrophoresis Southern blot analysis Western blot analysis DNA sequencing
Question 18 4 pts What role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes? Change the surface proteins of bacteria; since DNA is not protein, there is no need for protection Cut foreign DNA into pieces; bacteria have RNA genomes. Destroy invading viral DNA: bacterial DNA does not contain the restriction enzyme recognition sequences. Restrict the growth rate of bacteria; bacterial DNA is restriction enzyme resistant. Question 18 4...
Day 1, You have isolated DNA from 30 individuals and performed
restriction enzyme digestion. You have loaded the product and run
the gel electrophoresis following the instruction properly. You
expected to see DNA bands after the procedure is completed, like
figure A, but after the completion of the run your gel looks like
figure B. You did not see any DNA band. You were so upset, but your
lab partner fixed the problem and your gel looked like figure A....
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
1. A small piece of DNA was digested using restriction enzymes
HindIII and PstI:
A) How many basepairs long is the fragment?
a. 900 bp
b. 2000 bp
c. 600 bp
d. 500 bp
B) How many PstI restriction sites are located within
this fragment?
a. 0
b. 3
c. 1
d. 4
e. 2
Psti Pstl Hindili 500 500 100 900
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