Homework Answers
Answer: Restriction enzymes
Explanation:
Restriction enzymes are the enzymes used to cleave or cut the DNA at the specific sites to get the fragments of different size. These enzymes are actually the defense mechanism of the bacteria which is used to protect them from the phages. When the phages insert their DNA, these bacterial restriction enzymes cut them and make them inactive.
There are four types of restriction enzymes which is classified based on the recognition sequence, cleaving position etc. These enzymes frst recognise a specific sequence and make cut between specific nucleotides at the same site or near the site or away from the site on both the strands.
So these enzymes are used to make fragments of various sizes in this case.
Heat can denature the DNA but it cannot make fragments by cleaving or cutting at specific sites. DNases are the enzymes which are used to degrade the DNA. It is mainly used during the RNA isolation in order to get RNA free from DNA.
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Once the DNA has been collected from both a crime scene and suspect and amplified, what...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
is a nucleus the sole source of DNA in a eukaryotic cell? WORKSHEET: EXERCISE 10 NAME:...
is
a nucleus the sole source of DNA in a eukaryotic cell?
WORKSHEET: EXERCISE 10 NAME: DATE: SECTION Twenty microliters (20 ㎕) of each sample was loaded into your gel. Convert 2OuL to milliliters (mL). I. 2. Describe the charge on the DNA molecule and specify which component of the molecule is responsible for contributing to that charge. Why do some DNA samples travel further in the gel than others? 3. 4. Once electrophoresed, how are the DNA bands visualized?...
The PCR was a success and your target region of 770 bp in length has been...
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The PCR was a success and your target region of 440 bp in length has been amplified. You ligate a short linker containing an Apal restriction enzyme site onto both ends of the PCR product, dige...
The PCR was a success and your target region of 440 bp in length has been amplified. You ligate a short linker containing an Apal restriction enzyme site onto both ends of the PCR product, digest it with Apal, and clone it into the Apal site of the 5 kb plasmid diagrammed below amHI 300 EcoRI 5 kb 3400 1000 EcoRI 2000 Apal Draw the plasmid containing the cloned insert. Indicate clearly where the insert will be located. Include RE...
Please I need help on questions 1-4 in great detail please Load 15 mu l of...
Please I need help on questions 1-4 in great detail please
Load 15 mu l of the following samples from the above section onto the simple Wells. Seal the wells with agarose and electrophorese until the bromophenol blue in the samples has migrated to within 2 mm of the positive electrode end of the gel. Remove the gels from the unit and stain them as described in Section IV. Measure the distance of the DNA bands (in cm) from the...
When there is a match between a forensic DNA sample collected at a crime scene and...
When there is a match between a forensic DNA sample collected
at a crime scene and DNA subpoenaed from a suspect, the genotype
frequency can be interpreted in two ways. In one sense, it is the
probability of finding that specific genotype in the population. In
another sense it is the probability that the match is due to
chance.
How does the results from the most common alleles in
the different populations affect the application of using these
results for...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has...
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I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
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Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
is
a nucleus the sole source of DNA in a eukaryotic cell?
WORKSHEET: EXERCISE 10 NAME: DATE: SECTION Twenty microliters (20 ㎕) of each sample was loaded into your gel. Convert 2OuL to milliliters (mL). I. 2. Describe the charge on the DNA molecule and specify which component of the molecule is responsible for contributing to that charge. Why do some DNA samples travel further in the gel than others? 3. 4. Once electrophoresed, how are the DNA bands visualized?...
The PCR was a success and your target region of 770 bp in length has been amplified. You now plan to digest the DNA amplicon with the restriction enzyme Eael, and clone the resulting longest fragment it into the Eael site of the 5 kb plasmid diagrammed below. 770 bp BamHI 1 200 EcoRI 800 EcoRI 4000 1000 5 kb O /1000 2000 2000 Faal You purify your recombinant plasmid from bacterial cells, and run the plasmid (uncut. or not...
The PCR was a success and your target region of 440 bp in length has been amplified. You ligate a short linker containing an Apal restriction enzyme site onto both ends of the PCR product, digest it with Apal, and clone it into the Apal site of the 5 kb plasmid diagrammed below amHI 300 EcoRI 5 kb 3400 1000 EcoRI 2000 Apal Draw the plasmid containing the cloned insert. Indicate clearly where the insert will be located. Include RE...
Please I need help on questions 1-4 in great detail please
Load 15 mu l of the following samples from the above section onto the simple Wells. Seal the wells with agarose and electrophorese until the bromophenol blue in the samples has migrated to within 2 mm of the positive electrode end of the gel. Remove the gels from the unit and stain them as described in Section IV. Measure the distance of the DNA bands (in cm) from the...
When there is a match between a forensic DNA sample collected
at a crime scene and DNA subpoenaed from a suspect, the genotype
frequency can be interpreted in two ways. In one sense, it is the
probability of finding that specific genotype in the population. In
another sense it is the probability that the match is due to
chance.
How does the results from the most common alleles in
the different populations affect the application of using these
results for...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
The Ught Went Out on Sunshine In the quiet town of Utopla, 90 year-old Sunshine Moon had been murdered. Her murder had not only been brutal (her heart had been cut out), but also creepy. It appeared as if Sunshine's blood had been collected in a small sauce pan and tasted. The murder showed other signs of the occult: a candlestick and a pair of crossed pokers had been arranged near the body. Further investigation Indicated this was no supernatural...
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