Question

Indicate whether the following statements are true or false. Items (6 items) (Drag and drop into the appropriate area below) Protein folding releases energy to the surroundings (AG<0). All proteins are made of amino acids. All proteins require chaperones to fold. All proteins have disulfide bonds. Unfolded RNaseA cannot be refolded. "Molten globule" describes the initial stages of protein folding in which the conformation is still very dynamic. Categories True True False

Answer 1

1) ' Protein folding releases energy to surroundings ' is a true statement . Because it is known that protein folding is a spontaneous reaction ,then it must assume a negative Gibbs free energy.Gibbs free energy in protein folding is directly related to enthalpy and entropy .

2) 'All proteins are made up of amino acids'  is a true statement. Proteins are made up of small building blocks of amino acids, joined together in a chain.There are 20 different amino acids Some proteins are just a few amino acids chains while others are long chains of amino acids . These chains fold up in many different ways giving each protein a unique shapes.

3)' All proteins require chaperones to fold' is a false statement .Because most newly synthesized proteins can fold in absence of chaperones,a minority strictly require them for the folding .

4) 'All proteins have disulfide bond' is false statement .Because disulfide bond is often found in bacteria and eukaryotes .Some extracellular proteins also have several disulfide bonds.But it is lack in intracellular proteins . Usually these bonds form between the thiol groups of two cysteine amino acids .

5) 'Unfolded RNaseA cannot be refolded' is a false statement . Because it can be unfolded and refolded reversibly.

6) "'Molten globule" describe the initial stage of protein folding in which the conformation is still very dynamic' is a true statement. Molten globules are compact ,partially folded conformation of protein that have near native compactness,substantial secondary structure,little detectable tertiary structure.