Question

Your Name: 1. (continued) are provided with a tube containing an junknown unit concentration of β-galactosidase. You dilute the enzyme in the appropriate buffer and perforin enzyme assay in a total volume of 1.0 ml. Following 3 minutes incubation at 37°C, the following data was obtained after blanking the instrument with a tube not containing enzyme and assaying absorbance in a 1.0 ml cuvette. tube # β-galactosidase volume added 3 3 | 5 μ1 , s μ1-1-1 Abs@410 m 0.73 0.86 0.16 0.18 Calculate the äverage unit concentration of f-galactosidase for cach of these duplicate pairs. Show your work.

Answer 1

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