Homework Answers
The correct option is (b)
Silica has positively charged particles which has high affinity for the negatively charged DNA molecule. Under low salt concentration DNA molecules are separated & Under high salt conditions the DNA is tightly bound
Add Answer to:
QUESTION 18 conditions Silica Gel we have used for plasmid purification binds DNA in the presence...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
QUESTION 2 1. In the Qiagen DNA purification system that we used with EcoRi-cut Yeast genomic...
QUESTION 2 1. In the Qiagen DNA purification system that we used with EcoRi-cut Yeast genomic DNA in the last Lab A. Why is it necessary to "purify" the EcoRl-cut Yeast DNA before proceeding to the DNA "cloning" steps we are about to do today? (1 pt) B. Which major type(s) of macromolecule within the restriction digest/binding buffer (PB) mixture bind(s) to the resin in the Qiagen "spin column," and which type(s) flow(s) through? (0.5 ptea)
You have prepared some plasmid DNA in lab and need to know its concentration so you...
You have prepared some plasmid DNA in lab and need to know its concentration so you can load 1 ug on a gel. You dilute 1 uL with 99 uL of water and the resulting sample has an absorbance of 0.075. What volume of the original sample do you need to load onto the gel? (Show math for partial credit and make sure you are answering the question I am actually asking.)
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
Question 8 (8 pts) Certain reverse-phase stationary phases cannot be used under conditions of very low...
Question 8 (8 pts) Certain reverse-phase stationary phases cannot be used under conditions of very low pH. Use a clear and legible hand-drawing to show the following: a. The structure of a standard C4 group chemically bonded to a silica surface through a silyl ether bond. b. A reasonable mechanism by which acid hydrolysis (H+ and H20) might cleave the silyl ether bond, including the products of the reaction.
a) What is the total net charge of double-stranded DNA that is 500 basepairs (bp) in...
a) What is the total net charge of double-stranded DNA that is 500 basepairs (bp) in length? b) In agarose gel electrophoresis, two parallel and oppositely charged electrodes are situated at opposite ends of the gel. The surrounding solution is a “buffer”, an aqueous salt solution maintained at constant pH. The gel is made in the same buffer, with a small concentration of agarose. Because the concentration of agarose is small , everything between the electrodes can be considered to...
Question 18 When attempting to isolate a gene of interest, what is most commonly the first...
Question 18 When attempting to isolate a gene of interest, what is most commonly the first step? Not yet answered Points out of 1.00 Select one: a. gel electrophoresis b. enzyme digestion P Flag question c. DNA extraction d. protein purification e. PCR Question 19 What must a growth media contain? Not yet answered Points out of 1.00 Flag question Select one: a. a carbon source, such as glucose. b. sources of carbon, nitrogen, phosphorus, and various trace metals c....
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...
QUESTION 2 1. In the Qiagen DNA purification system that we used with EcoRi-cut Yeast genomic DNA in the last Lab A. Why is it necessary to "purify" the EcoRl-cut Yeast DNA before proceeding to the DNA "cloning" steps we are about to do today? (1 pt) B. Which major type(s) of macromolecule within the restriction digest/binding buffer (PB) mixture bind(s) to the resin in the Qiagen "spin column," and which type(s) flow(s) through? (0.5 ptea)
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
Question 8 (8 pts) Certain reverse-phase stationary phases cannot be used under conditions of very low pH. Use a clear and legible hand-drawing to show the following: a. The structure of a standard C4 group chemically bonded to a silica surface through a silyl ether bond. b. A reasonable mechanism by which acid hydrolysis (H+ and H20) might cleave the silyl ether bond, including the products of the reaction.
Question 18 When attempting to isolate a gene of interest, what is most commonly the first step? Not yet answered Points out of 1.00 Select one: a. gel electrophoresis b. enzyme digestion P Flag question c. DNA extraction d. protein purification e. PCR Question 19 What must a growth media contain? Not yet answered Points out of 1.00 Flag question Select one: a. a carbon source, such as glucose. b. sources of carbon, nitrogen, phosphorus, and various trace metals c....
Most questions answered within 3 hours.
-
To buy a new house you must borrow $155,000. To do this you take
out a...
asked 1 minute ago -
Spacely Sprockets is evaluating the construction of a new plant
on land the company purchased for...
asked 50 minutes ago -
1. Consider a linear regression model of y on K regressors and
an intercept.
(i) Describe...
asked 51 minutes ago -
Enter a balanced equation for the reaction between hydrochloric
acid and sodium sulfite.
Express your answer...
asked 56 minutes ago -
Give a regular expression describing the language
{x | x ∈ Σ* and x does not...
asked 57 minutes ago -
Masses of 1.0 kg, 2.0 kg, and 3.0 kg are each separately subject
to a net...
asked 1 hour ago -
The mode of philosophical argumentation and thought. How do
philosophers think and write? What is important...
asked 1 hour ago -
At the beginning of the unit, you were asked whether you thought
it was appropriate or...
asked 1 hour ago -
Calculate the grams of carbon in 9.32 x 10+23 molecules of
benzene (C6H6).
asked 1 hour ago -
A 5-year Treasury bond has a 4.95% yield. A 10-year Treasury
bond yields 6.6%, and a...
asked 1 hour ago -
suppose you own a chain of five restaurants in
downtown Vancouver. as the manager, you want...
asked 1 hour ago -
Here are summary statistics for randomly selected weights of
newborn girls:
nequals=225x overbarxequals=28.7hg, sequals=7.5hg. Construct a...
asked 1 hour ago