Question

Which type of transcription factor would bind to a DNA segment with the following sequence? What is the structure of the DNA binding domain of the protein? What molecular events does binding of the protein promote?

5’ TATATATA 3’

3’ ATATATAT 5’

Answer 1

Transcription factors are the proteins which bind to sepecific region of DNA & initiate and regulate the process of Transcription

The following segment of DNA given in the question contain TATA sequence and Transcription factor which bind to this sequence is one of the Generalized Transcription factor called Transcription factor II D which helps in recruiting polymerase & other transcription factor to core promoter

It is composed of 11 subunit

1 subunit is Tata binding protein ( TBP) which contain beta sheet domain motif and interact at minor groove of DNA

10 subunit are of TBP associated factors TAFs

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When this protein bind to TATA sequence the TBP subunit having $\beta$ sheet motif interact to Minor grrove of DNA and bend it to 90o angle which Double stranded DNA to single stranded DNA

And it recruits RNA polymerase and other transcription factors initiating process of Transcription

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