Question

Glycine COO H3N'-C-H

Answer 1

1. pH of stacking gel = 6.8

pH of resolving gel = 8.8
pH of the buffer used to run the gel = 8.3
pI of glycine = 6.06
pK1 of glycine = 2.34
pK2 of glycine = 9.60
So,
i. Glycine exhibits +1 positive charge at pH below pK1.
ii. Glycine exhibits neutral charge at pH = pI.
i. Glycine exhibits -1 negative charge at pH above pK2.

NH»- CoO COO At P 6.ob Tn Stactirg gel

2. 5 mL of 5% acrylamide gel

For 1% gel -----> 1 g in 100 mL

For 5% gel -----> 5 g in 100 mL

For 5% gel -----> 0.25 g in 5 mL

3. 100 mL of 10X buffer is given.

1 mL of 100 X buffer + 99 mL of water = 100 mL of 1 X buffer