Question

Can you help me to write hypothesis and prediction for this and also help me answer this I made a graph but I’m confused .

Experiment B.The Effect of pH on Amylase Activity 6 standard ses pht paper daponable pasteur pipeties Introduction in this experimett (Check the Introduction to ๑uestion Pose a question about plt and reaction raie. Hypothesis Hypothesine about the rate of activity of amylase at various P Enzymes Prediction Predict the results of the experiment based on your hypothesis (if/then). Procedure 1. Using a wax pencil, number six standard test tubes 1 through i Begining with tube 1 and pH 4, mark one tube for each pH of buffer (4,5,6,7,8,9 After you mark the test tubes, use a 5-ml graduated pipette to add 5 mL of the appropriate buffer to each test tube (5 mL buffer 4 to tube 1.5 ml buffer 5 to tube 2, etc.). Rinse the pipette with distilled water after con bun skinl Avoid contact with all solutions. Do not by mouth. Wosh hands thoroughly after each ccurs notity the instructor itf the instructor is dsposable gloves and use dry paper towels to dry towels with towels soaked in soop ond owels in the trash

Answer 1

Hypothesis: The enzyme amylase has a maximum activity and optimum pH in a range of 3-9.

Prediction: If the amylase activity is found to be maximum in the pH range 3-9, then its optimum pH lies within that pH range.

From the given results, the starch disappearance is maximum in the pH range of 6- 7. Less time for the starch disappearance is in the pH 6-7. Hence the eznyme shows maximum activity in this pH range. Thus the optimum pH of amylase activity lies in this pH range. The first graph shows the time for starch disappearance (y axis) vs. pH (x-axis).

The second graph shows the activity (the unit and numbers are not given) of enzyme vs. pH.

Note: Hypothesis is somethaing that you expect the results from the experiment. And prediction always makes statement using if..then. This is what you observe the outcome if the hypothesis is supported.