Question

What type of graph should I make for the data in table 4? Please provide an example.

Wina marker, label seven test tubes with your initials, and pR2, 14- With a china mar 12 the tubes to the location of the phosphate buffer series and fill each tube with 2 ml of the corresponding ere fill the tube marked pH 2 with 2 ml of the buffer marked pH 2 etc.). Use the graduated cylinders to add the buffers. Return to your work area and add 1 ml of potato extract to each of the seven tubes. Agitate the tubes by hand. Table 4: Effect of pll on Enzyme Activity Color Intensity Add 1 ml of 1% catechol to each of the seven tubes. Cap the tubes and mix contents. Place the tubes in the 40°C water bath. Mix the tubes periodically over the next 10 min. pH 2 pH 4 pH 6 Tp pH 8 DH 10 . After 10 min. remove the tubes from the water bath and record the color intensity of each tube's content in table 4. pH 12 1) Over what pH range does catechol oxidase catalyze catecholto benroquinone? 2) What is the optimum pil for catechol oxidase activity? Homework: Based on data summarized in Table 4, make a graph expressing the activity of catechol oxidase as a function of variation in pH. Remember write a figure caption and your hypothesis/prediction with the graph.

Answer 1

The graph generally have pH on X axis and enzyme activity on Y axis.

Since the intensity of colour is given, we can see that the intensity of colour increases with the increase in pH but then it is decreasing. Then from here it can be said that the optimum pH will be between 7 and 8. Since from here the enzyme activity cannot be obtained, a graph with colour intensity and pH can be plotted.

colour + intensity ) G