What makes a plasmid a conjugative plasmid
Homework Answers
A plasmid becomes a conjugative plasmid when it contains plasmids that are capable of transferring themselves to the other bacteria. F plasmid is an example of a conjugative plasmid where the male cell or the F+ cell that has the plasmid will replicate itself and transfer it to a F- or a female cell by the means of a pilus which is a long hollow tube that physically connects the two bacterial cells.
Question 2 (0.2 points) A plasmid that encodes all of the proteins necessary for transferring a...
Question 2 (0.2 points) A plasmid that encodes all of the proteins necessary for transferring a copy of the plasmid into a recipient cell is plasmid. a) integrated b) insertion c) restriction d) conjugative e) replicative
staring from Plasmids: I need the definitions Generalized transduction: Specialized transduction: Plasmids: Conjugative plasmid: Dissimilation plasmid:...
staring from Plasmids: I need the definitions
Generalized transduction: Specialized transduction: Plasmids: Conjugative plasmid: Dissimilation plasmid: Plasmids that code for proteins that enhance pathogenicity: E. coli: S. aureus: Clostridium tetani: Bacillus anthracis: Bacteriocins: Resistance factors (R factors): Resistance transfer factor (RTF): r-determinant: Transposons: Insertion sequences (IS): Transposase: Recognition sites: Complex transposons:
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
What is the function of 3X FLAG in a plasmid?
What is the function of 3X FLAG in a plasmid?
You are working with a plasmid the has a copy number of 3. What are the...
You are working with a plasmid the has a copy number of 3. What are the chances that one of the daughter cells (either one) will be cured of this plasmid (that is, will not have any plasmid in it?). Provide your answer as THE DENOMINATOR of the fraction such as "200" if the answer is 1/200, or "4" if the answer is 1/4.
1. Why do plasmid vectors contain a polylinker? So that the plasmid can be cut into...
1. Why do plasmid vectors contain a polylinker? So that the plasmid can be cut into many small fragments to be inserted in the region of interest To allow the plasmid to survive on antibiotic media To find a restriction enzyme site that is useful for inserting the fragment of interest Multiple cut sites are required for the DNA fragment to be inserted. 2.What does blue color mean in blue-white screening with pUC18? The DNA fragment was successfully inserted into...
1. What are some reasons for isolating plasmid DNA? 2. What are the functions of sodium...
1. What are some reasons for isolating plasmid DNA? 2. What are the functions of sodium hydroxide and SDS in the cell lysis solution? What is the function of the potassium acetate solution? 3. What structural property of plasmid DNA allows it to be separated from chromosomal DNA during alkaline cell lysis? Was more than one band observed in your plasmid sample after electrophoresis and staining?
What is the purpose of the neutralization solution for a miniprep of plasmid DNA?
What is the purpose of the neutralization solution for a miniprep of plasmid DNA?
The Cas9 plasmid contains a kanamycin resistance gene and the gRNA plasmid contains an ampicillin resistance...
The Cas9 plasmid contains a kanamycin resistance gene and the gRNA plasmid contains an ampicillin resistance gene. What was the purpose of adding these genes to the plasmids during the CRISPER procedure?
Question 1 - 12 What does the AMPr gene in the PGEM-T plasmid produce and what...
Question 1 - 12 What does the AMPr gene in the PGEM-T plasmid produce and what is the role of this product in the protocol itself? Type your answer Question 2 --12 What does the lacZ gene in the PGEM- T plasmid produce and what is the role of this product in the protocol itself? Type your answer Question 3 (413) What occurs during the ligation step of Protocol 11? Type your answer Question 4 ( -- /2 What is...
Question 2 (0.2 points) A plasmid that encodes all of the proteins necessary for transferring a copy of the plasmid into a recipient cell is plasmid. a) integrated b) insertion c) restriction d) conjugative e) replicative
staring from Plasmids: I need the definitions
Generalized transduction: Specialized transduction: Plasmids: Conjugative plasmid: Dissimilation plasmid: Plasmids that code for proteins that enhance pathogenicity: E. coli: S. aureus: Clostridium tetani: Bacillus anthracis: Bacteriocins: Resistance factors (R factors): Resistance transfer factor (RTF): r-determinant: Transposons: Insertion sequences (IS): Transposase: Recognition sites: Complex transposons:
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
1. What are some reasons for isolating plasmid DNA? 2. What are the functions of sodium hydroxide and SDS in the cell lysis solution? What is the function of the potassium acetate solution? 3. What structural property of plasmid DNA allows it to be separated from chromosomal DNA during alkaline cell lysis? Was more than one band observed in your plasmid sample after electrophoresis and staining?
Question 1 - 12 What does the AMPr gene in the PGEM-T plasmid produce and what is the role of this product in the protocol itself? Type your answer Question 2 --12 What does the lacZ gene in the PGEM- T plasmid produce and what is the role of this product in the protocol itself? Type your answer Question 3 (413) What occurs during the ligation step of Protocol 11? Type your answer Question 4 ( -- /2 What is...
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