Homework Answers
pUC 19 has LacZa gene and produces blue colonies on exposure to X-gal. The blue colonies are formed because of the presence of LacZ gene. But it doesn't have resiso to both ampicillin and tetracycline.
pBR322 has resistance to both ampicillin and tetracycline. But it doesn't contain LacZa gene and doesn't produce blue colonies as it doesn't have LacZ gene.
Hope this helps, Good luck!!!
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Asap, please.
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Please answer these four questions.
6C. A type of plasmid used frequently as a vector in recombinant DNA procedures carries gene markers for resistance to tetracycline (Tet') and to ampicillin (Amp). In one experiment, such plasmids were exposed to a restriction enzyme that cuts within the Amp' gene but leaves the Tet' gene intact. Plasmids exposed to the enzyme were next mixed with genomic restriction fragments from a cloned library of mouse DNA. The aim was to produce and detect...
4. The DNA profiles to the right below represent four different individuals. o 111 Which of the following statements is consistent with the results? A. B is the child of A and C. B. C is the child of A and B. C. D is the child of B and C. D. A is the child of B and C. E. A is the child of C and D. <ULTE ILI UTILIT THITI TIL ILI 5. A eukaryotic gene with...
Please explain the following question and answer all
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2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
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Protein P is synthesized in relatively high amounts in the human pancreas. This protein has been isolated and purified, but its amino acid sequence has not been determined. We wish to clone the gene for protein P. (a) How can a probe be prepared to identify the gene for protein P? (b) If we have prepared a radioactive messenger RNA as our probe in part (a), how could we verify that it is the mRNA for protein P? (c) If...
Asap, please.
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