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1. Why is it important that ATP synthase functions in both ATP synthesis mode and in reverse?
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ATP synthase is an enzyme which helps in production of ATP which is an energy molecule. The enzyme helps in formation of ATP from ADP and Pi. The ATP synthase works for in ATP synthesis mode and reverse because the production of ATP from ADP + Pi is energetically unfavorable and hence needs to move in reverse direction. Hence for the enzyme to drive this reaction, it has to couple with cellular respiration due to an electrochemical gradient that is formed by proton difference across the mitochondrial membrane. It acts in ATP synthesis mode for the production of the ATP and in reverse mode so that it gets energy for the production of ATP from ADP and Pi

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